Abstract
The competitiveness of bacterial cellulose (BC) production with plant cellulose can be achieved by production on cost-effective media. It was found that the bacterial cell number ratio of BC to culture medium increases over time so that from the fourth day, the entrapped cell number in the cellulose network exceeds the suspended cells. Optimization based on 23-full factorial showed that inoculum development at 50 rpm and the main culture process under static conditions significantly increases BC production. A cost-effective culture medium containing molasses (ML) and corn steep liquor (CSL) was developed based on the same C/N ratio to HS medium, with 7.24 g/l cellulose at C/N ratio 12.6 is competitive with maximum production 8.7 g/L in HS medium. The BC production cost was reduced about 94% using the proposed cheap and locally available medium containing ML and CSL, while BC mechanical properties increased by about 50%.
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The all of generated data during the current study are available in this article.
Abbreviations
- C:
-
Carbon
- N:
-
Nitrogen
- RSM:
-
Response surface methodology
- BC :
-
Bacterial cellulose
- BBD:
-
Box-Behnken design
- Eth:
-
Ethanol
- CSL:
-
Corn steep liquor
- HS:
-
Hestrin-Schramm
- DOE:
-
Design of experiment
- ML:
-
Molasses
- Glu:
-
Glucose
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Valiollah Babaeipour has designed and directed this research. Motahareh Rouhi has conducted experiments under supervisor Valiollah Babaeipour. Sirwan Khanchezar has written the manuscript. All authors have read and approved the manuscript.
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Rouhi, M., Khanchezar, S. & Babaeipour, V. Economical Optimization of Industrial Medium Culture for Bacterial Cellulose Production. Appl Biochem Biotechnol 195, 2863–2881 (2023). https://doi.org/10.1007/s12010-022-04239-2
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DOI: https://doi.org/10.1007/s12010-022-04239-2