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Exo-III Enzyme and DNAzyme-Assisted Dual Signal Recycles for Sensitive Analysis of Exosomes by Using Personal Glucose Meter

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Abstract

Exosome plays a crucial role in regulating intercellular communication during atherosclerosis development. However, sensitive and portable exosome detection remains a huge challenge. Herein, a personal glucose meter (PGM)-based exosomes detection approach has been proposed that allows detection of exosomes with a high sensitivity and reproducibility. In this method, a catch probe, which is composed of CD63 aptamer and blocker sequence, is utilized for the specific identification of exosomes. The blocker sequence binds with H probe to initiate the Exo-III-assisted signal recycles to generate numerous DNAzyme sequences. Under the assistance of the substrate, DNAzyme forms its active secondary structure to generate gap site in substrate, releasing a linker to conjugate sucrase to streptavidin magnetic beads (SMBs). After removing unbound sucrase, the SMB-linker-sucrase complex is used to catalyze sucrose to glucose, which can be read by PGMs. Based on this, the method exhibits a wide detection range and a low limit of detection, holding a promising prospect for the analysis of exosomes and screening atherosclerosis.

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Acknowledgements

The authors thank the financial and technical support from The Third Affiliated Hospital of Chongqing Medical University.

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Authors and Affiliations

  1. Department of Cardiology, 958 Hospital of PLA Army, Chongqing, (400020), China

    Wenyi Hu

  2. Department of Cardiology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, (401120), China

    Hailong Su, Xiaojuan Zeng, Xinglian Duan, Yangqiu Li & Li Li

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  1. Wenyi Hu
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  2. Hailong Su
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  3. Xiaojuan Zeng
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  4. Xinglian Duan
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  5. Yangqiu Li
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  6. Li Li
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Contributions

L.L financed the research. H.W. and L.L designed and wrote the manuscript. H.W. performed experiments. S.H, Z.X., D.X. and L.Y. assisted data analysis.

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Correspondence to Li Li.

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Hu, W., Su, H., Zeng, X. et al. Exo-III Enzyme and DNAzyme-Assisted Dual Signal Recycles for Sensitive Analysis of Exosomes by Using Personal Glucose Meter. Appl Biochem Biotechnol 195, 861–870 (2023). https://doi.org/10.1007/s12010-022-04171-5

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  • DOI: https://doi.org/10.1007/s12010-022-04171-5

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