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Co-expression of Xylose Transporter and Fructose-Bisphosphate Aldolase Enhances the Utilization of Xylose by Lactococcus lactis IO-1

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Abstract

The raw material cost of lactic acid fermentation accounts for the main part of the production cost, and this necessitates the exploration of the efficient use of cheap raw materials in lactic acid production. We compared the outcomes of the homologous expressions of xylose transporters (xylFGH, xylE, araE, and xylT), 6-phosphofructokinase (pfkA), fructose-bisphosphate aldolase (fbaA), and their co-expression in Lactococcus lactis IO-1 on lactic acid production using xylose as the raw material. We found that the production rate of lactic acid on xylose fermentation by L. lactis IO-1 overexpressing fbaA was the highest (14.42%). Among the xylose transporters investigated, XylT had the strongest xylose transport capacity in L. lactis IO-1, with an increase in the lactic acid production rate by 10.38%. The genes near the overexpression of fbaA or xylT in the metabolic pathway were more upregulated than the distant genes. The co-expression of fbaA and xylT increased the production rate of lactic acid by 27.84% on xylose fermentation by L. lactis IO-1. This work presents a novel strategy for the simultaneous enhancement of the expression of important genes at the beginning and midway of the xylose metabolic pathway of L. lactis IO-1, which could greatly improve the target production.

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Funding

This work was supported by the National Natural Science Foundation of China (grant number 31200023; 22008083), and the Natural Science Foundation of Huaian City (HAB202054).

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Authors and Affiliations

  1. Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, 1 Meicheng Road, Huaian, 223003, Jiangsu Province, China

    Yejuan Qiu, Hanwen Zhang, Yuxiang Yang, Wenyi Hou & Xiangqian Li

  2. Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, 111 Changjiang West Road, Huaian, 223300, Jiangsu Province, China

    Zhongyang Qiu, Jun Xia, Xiaoyan Liu & Jianlong He

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Contributions

Material preparation, data collection, and analysis were performed by Hanwen Zhang, Yuxiang Yang, and Wenyi Hou. Yejuan Qiu, Xiangqian Li, and Jianlong He designed the experiments and wrote the manuscript. Zhongyang Qiu, Jun Xia, and Xiaoyan Liu commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Xiangqian Li or Jianlong He.

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Qiu, Y., Qiu, Z., Xia, J. et al. Co-expression of Xylose Transporter and Fructose-Bisphosphate Aldolase Enhances the Utilization of Xylose by Lactococcus lactis IO-1. Appl Biochem Biotechnol 195, 816–831 (2023). https://doi.org/10.1007/s12010-022-04168-0

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