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Fermentative production of poly-β-hydroxybutyric acid from xylose by a two-stage culture method employing Lactococcus lactis IO-1 and Alcaligenes eutrophus

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Summary

A two-stage culture method employing Lactococcus.lactis IO-1 and Alcaligenes eutrophus was developed for production of poly-β-hydroxybutyric acid (PHB) from xylose. In this method, xylose was converted to L-lactic acid and acetic acid by L.lactis IO-1, and then the organic acids were converted to PHB by A.eutrophus. When the supernatant of the IO-1 culture broth, containing 10 g·dm-3 L-lactate derived from xylose, was used as medium for A. eutrophus, the concentration of cells increased to 8.5 g·dm-3 in 24 h and 55% of the content in the cells by weight was PHB.

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Authors and Affiliations

  1. Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Hakozaki, Higashi-ku, 812, Fukuoka, Japan

    Kenji Tanaka, Kouji Katamune & Ayaaki Ishizaki

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  1. Kenji Tanaka
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  2. Kouji Katamune
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  3. Ayaaki Ishizaki
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Tanaka, K., Katamune, K. & Ishizaki, A. Fermentative production of poly-β-hydroxybutyric acid from xylose by a two-stage culture method employing Lactococcus lactis IO-1 and Alcaligenes eutrophus . Biotechnol Lett 15, 1217–1222 (1993). https://doi.org/10.1007/BF00130300

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