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Resource Utilization of Bovine Neck Ligament: Enzymatic Preparation of Elastin Peptide and Its Antioxidant Activity

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Abstract

Elastin is considered an excellent resource for obtaining antioxidant peptides due to unique amino acid composition. However, it is hardly soluble in water or in dilute acid or alkali; most of the elastases have low yields for preparing elastin peptides, making it difficult to meet industrial applications. To address above problems, enzymes capable of hydrolyzing elastin into soluble peptides were preferred from typical commercial protease preparations. The optimal enzymatic hydrolysis process conditions for elastin peptides were obtained by response surface optimization design. The molecular weight, amino acid composition, and antioxidant activity of the enzymatic hydrolysis products were determined. The results show that the alkaline protease NUE has a strong hydrolysis effect. The optimized enzymatic hydrolysis conditions are as follows: substrate concentration is 5%, enzyme concentration is 650 U/mL, pH is 10.0, temperature is 60 °C, time is 6 h. The degree of hydrolysis of elastic protein peptides obtained through this method is 14.42%. The distribution of molecular weight is 200–6500 Da, more than 85% of the component molecular amount is greater than 800 Da; the amino acid content related to antioxidant activity has reached 68 mg/100 mg, so it has extremely high free radical clearance. Compared with acid and alkali methods, the anti-oxidation capacity of enzyme-based peptide is better, the reaction conditions are milder, the yield is higher, and by-products and pollutants are fewer. It provides an effective way to industrialized production of elastin peptides with high antioxidant activity and a basis for its widespread application in the food and pharmaceutical industries.

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All data and materials generated are included in this published article and are available upon reasonable request.

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Acknowledgements

The authors would like to thank teacher at the Laboratory of Leather Chemistry and Engineering Center. This work was financially supported by National Key Research and Development Program of China (2017YFB0308402).

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Authors and Affiliations

  1. National Engineering Research Center for Clean Technology in Leather Industry, Sichuan University, Chengdu, 610065, People’s Republic of China

    Qiao Sun, Xu Zhang, Mengchu Gao, Chunxiao Zhang & Biyu Peng

  2. Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu, 610065, People’s Republic of China

    Qiao Sun, Xu Zhang, Mengchu Gao, Chunxiao Zhang & Biyu Peng

  3. College of Biomass Science and Engineering, Sichuan University, Chengdu, 610065, People’s Republic of China

    Biyu Peng

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  1. Qiao Sun
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  2. Xu Zhang
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  4. Chunxiao Zhang
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  5. Biyu Peng
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Contributions

Qiao Sun: conceptualization, methodology, validation, writing—review and editing. Xu Zhang: data curation. Mengchu Zhang: investigation, formal analysis. Chunxiao Zhang: investigation. Biyu Peng: resources, conceptualization, supervision, funding acquisition.

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Correspondence to Biyu Peng.

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Sun, Q., Zhang, X., Gao, M. et al. Resource Utilization of Bovine Neck Ligament: Enzymatic Preparation of Elastin Peptide and Its Antioxidant Activity. Appl Biochem Biotechnol 195, 33–50 (2023). https://doi.org/10.1007/s12010-022-04102-4

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  • DOI: https://doi.org/10.1007/s12010-022-04102-4

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