Abstract
The potential of bioethanol generation using the mixture of waste French fries (WFF) and municipal wastewater (MWW) via separate hydrolysis and fermentation (SHF) was evaluated in this study. The effect of WFF substrate loading (SL, 10%, 16%, and 20%, w/v) on the SHF was also examined. Both glucose production and hydrolysis efficiency increased with increasing of SL from 10 to 16% and the maximum glucose yield of 0.236 g glucose/g WFF and hydrolysis efficiency of 91.9% were obtained at SL of 16%. However, the glucose production and hydrolysis efficiency decreased when the SL further increased to 20% due to the inhibition on enzyme caused by higher glucose production. The mixture hydrolysate was then used as feedstock for ethanol fermentation. The maximum ethanol production of 22.69 g/L was obtained from SL of 16%. The highest rate of glucose conversion to ethanol was 84.2%. The results demonstrated that the mixture of WFF and MWW could be used for ethanol production by the SHF.
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Funding
This work was supported by National Key Research and Development Program-International Cooperation Project (2019YFE0124600); Zhejiang Education Department and Research Foundation from Hangzhou Dianzi University (2021R407075).
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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by X Chen, X Zheng, and Y Pei. The first draft of the manuscript was written by X Chen and W Han. J Huang, J Tang, P Hou, and W Han commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Chen, X., Zheng, X., Pei, Y. et al. Ethanol Production from the Mixture of Waste French Fries and Municipal Wastewater via Separate Hydrolysis and Fermentation. Appl Biochem Biotechnol 194, 6007–6020 (2022). https://doi.org/10.1007/s12010-022-04084-3
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DOI: https://doi.org/10.1007/s12010-022-04084-3