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Evaluation of Prebiotic Properties of Galactooligosaccharides Produced by Transgalactosylation Using Partially Purified β-Galactosidase from Enterobacter aerogenes KCTC2190

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Abstract

Transgalactosylation reaction is the penultimate step in the production of galactooligosaccharides (GOSs) which has prominent applications in the treatment of disorders. In the present study, partially purified β-galactosidase from Enterobacter aerogenes KCTC2190 was used for the synthesis of prebiotic GOSs. GOSs were produced using lactose as substrate. Structural elucidation of collected fractions of GOSs by liquid chromatography electrospray ionization mass spectrometry exhibited the appearance of major peaks of produced GOSs at m/z 241.20, 481.39, 365.11, 527.17, and 701.51 respectively. GOSs facilitated the growth of potential probiotic strains (Lactobacillus delbrueckii ssp. helveticus, Bifidobacterium bifidum, and Lactiplantibacillus plantarum) and liberated propionate and butyrate as principal short-chain fatty acids which established its prebiotic potency. Synbiotic combinations exhibited good antioxidant activities. Synbiotic combinations also exhibited antimicrobial activities against pathogenic microorganisms namely Staphylococcus aureus and Escherichia coli. Synbiotic combinations of GOSs and the respective probiotic microorganisms were able to decrease viable human bone cancer cells (MG-63).

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Acknowledgements

The authors are grateful to the Indian Council of Medical Research, New Delhi, India, for providing Senior Research Fellowship. Authors are also thankful for the facilities provided by School of Community Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, India, to complete the work.

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Authors and Affiliations

  1. School of Community Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, India

    Manisha Maity, Sayari Majumdar, Dipak K. Bhattacharyya & Jayati Bhowal

  2. Center of Healthcare Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, India

    Ankita Das & Ananya Barui

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  1. Manisha Maity
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Correspondence to Jayati Bhowal.

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Maity, M., Majumdar, S., Bhattacharyya, D.K. et al. Evaluation of Prebiotic Properties of Galactooligosaccharides Produced by Transgalactosylation Using Partially Purified β-Galactosidase from Enterobacter aerogenes KCTC2190. Appl Biochem Biotechnol 195, 2294–2316 (2023). https://doi.org/10.1007/s12010-022-04073-6

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