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Biosynthesis of Silica Nanoparticles Using the Leaf Extract of Punica granatum and Assessment of Its Antibacterial Activities Against Human Pathogens

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Abstract

Plant-mediated nanoparticle synthesis is a revolutionary technique with numerous applications in fields, such as agriculture, food processing, and medicine. This study reports that Punica granatum leaf extract is capable of the green and eco-friendly synthesis of silica nanoparticles that provides a simple, cost-effective, and efficient methodology. P. granatum leaf extract was employed as a capping and stabilizing agent for the formation of silica nanoparticles, which were synthesized by a biological method using tetra ethyl ortho silicate. Biosynthesized silica nanoparticles are characterized by X-ray diffraction analysis, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy. All the analyses and characterization determined that the particles were spherical in shape and amorphous in nature with an average size of 12 nm. P. granatum-assisted silica nanoparticles were tested for antibacterial activity by a well-diffusion method against two-gram negative bacterial pathogens (E. coli and Salmonella sp.). The antibacterial studies prove that P. granatum-assisted silica nanoparticles have good antibacterial properties. These studies will help us find a new nano-drug or medicine against multidrug-resistant bacteria.

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Acknowledgements

The authors thank the Karpagam Academy of Higher Education for providing its laboratory facilities to conduct the experiments and also acknowledge the DST-FIST for supporting the infrastructure facility (SR/FST/LS-1/2018/187).

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Authors and Affiliations

  1. Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore, 641021, Tamil Nadu, India

    Rajiv Periakaruppan, Manju Praveena S, Priya C, Ranjitha P & Gokul Raj S

  2. Centre for Ocean Research (DST-FIST Sponsored Centre), MoES - Earth Science and Technology Cell (Marine Biotechnological Studies), Sathyabama Institute of Science and Technology, Col. Dr. Jeppiaar Research Park, Chennai, 600 119, Tamil Nadu, India

    Jeyapragash Danaraj

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  1. Rajiv Periakaruppan
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  2. Manju Praveena S
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  3. Priya C
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  4. Ranjitha P
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  5. Gokul Raj S
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Contributions

PR: Supervision, funding acquisition, and project administration

MPS: Investigation

PC: Methodology

RP: Data curation and writing—original draft preparation

GRS: Data curation and writing—original draft preparation

JD: Data curation and writing

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Correspondence to Rajiv Periakaruppan.

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Periakaruppan, R., S, M.P., C, P. et al. Biosynthesis of Silica Nanoparticles Using the Leaf Extract of Punica granatum and Assessment of Its Antibacterial Activities Against Human Pathogens. Appl Biochem Biotechnol 194, 5594–5605 (2022). https://doi.org/10.1007/s12010-022-03994-6

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  • DOI: https://doi.org/10.1007/s12010-022-03994-6

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