Abstract
Biofilms formed by bacteria are the group of sessile microbial cells that remain encompassed by self-secreted polymeric substances and have resulted in great health-care concern. The extracellular polymeric substances (EPS) prevent the penetration of antibiotics and other drugs, thereby resulting in the development of multi-drug resistance or antibiotic resistance. The biofilm-associated prosthetics being places at the joins of bone injury are the common sites for the development of biofilm-associated infection. This often spreads and results in the development of orthopaedic infections. Most of the infections are associated with musculoskeletal system and originate from non-living surfaces. The biofilm prevents the penetration of drugs, thereby resulting in the development of antibiotic resistance or multi-drug resistance. The minimum inhibitory concentration (MIC) for allicin and quercetin was found to be 80 µg/mL for quercetin and 100 µg/mL for amoxicillin against the sessile communities of Pseudomonas aeruginosa associated with the orthopaedic infection. The role of quercetin and allicin in reduction of protein, carbohydrate and eDNA content of the exopolysaccharides (EPS) was tested. The anti-quorum sensing activity of quercetin and allicin was confirmed both by biochemical and by photomicrographic studies. The antibiofilm and antimicrobial activities of quercetin and allicin were determined both by in vitro and in silico studies on P. aeruginosa bacterial strain from biofilm-associated orthopaedic infection.
Similar content being viewed by others
Data Availability
Not applicable.
References
Jamil, N., & Pa’ee, F. (2002) Antimicrobial activity from leaf, flower, stem, and root of Clitoria ternatea – A review. AIP Conference Proceedings 1:020044. doi:10.1063/1.5050140
Tomaz, I., Maslov, L., Stupić, D., Preiner, D., Ašperger, D., & Kontić, J. K. (2015). Multi-response optimisation of ultrasound-assisted extraction for recovery of flavonoids from red grape skins using response surface methodology. Phytochemical Analysis, 27(1), 13–22.
Victor, A., David, A., Arulmoli, R., & Parasuraman, S. (2016). Overviews of biological importance of quercetin: A bioactive flavonoid. Pharmacogn Rev., 10(20), 84–89.
Ouyang, J., Sun, F., Feng, W., & Chen, Y. (2016). Quercetin is an effective inhibitor of quorum sensing, biofilm formation and virulence factors in Pseudomonas aeruginosa. Journal of Applied Microbiology, 120(4), 966–974.
Hudzicki, J. (2009) Kirby-bauer disk diffusion susceptibility test protocol.
Gandhi, A. D., Vizhi, D. K., Lavanya, K., Kalpana, V. N., Devi Rajeswari, V., & Babujanarthanam, R. (2007). In vitro anti-biofilm and anti-bacterial activity of Sesbania grandiflora extract against Staphylococcus aureus. Biochem. Biophys. Rep., 12, 193–197.
Teanpaisan, R., Kawsud, P., Pahumunto, N., & Puripattanavong, J. (2016). Screening for antibacterial and antibiofilm activity in Thai medicinal plant extracts against oral microorganisms. Journal of Traditional & Complementary Medicine, 7(2), 172–177.
Brunk, C. F., Jones, K. C., & James, T. W. (1979). Assay for nanogram quantities of DNA in cellular homogenates. Analytical Biochemistry, 92(2), 497–500. https://doi.org/10.1016/0003-2697(79)90690-0
Oh, S. J., Kim, Y. S., Kwon, C. W., Park, H. K., Jeong, J. S., & Kim, J. K. (2009). Overexpression of the transcription factor AP37 in rice improves grain yield under drought conditions. Plant Physiology, 150(3), 1368–1379. https://doi.org/10.1104/pp.109.137554
Andersson, R., Enroth, S., Rada-Iglesias, A., Wadelius, C., & Komorowski, J. (2009). Nucleosomes are well positioned in exons and carry characteristic histone modifications. Genome Research, 19(10), 1732–1741.
Vipin C.,Mujeeburahiman M., Saptami K., Arun A.B. , Rekha P.D. (2019). Synergistic interactions of quercetin with antibiotics against biofilm associated clinical 2 isolates of Pseudomonas aeruginosa in vitro bioRxiv preprint. doi: https://doi.org/10.1101/601336.
Gopu, V., Meena, C. K., & Shetty, P. H. (2015). Quercetin influences quorum sensing in food borne bacteria: In-vitro and in-silico evidence. PloS One., 10, e0134684.
Jachlewski, S., Jachlewski, W. D., Linne, U., Bräsen, C., Wingender, J.,Siebers, B. (2015). Isolation of Extracellular Polymeric Substances from Biofilms of the Thermoacidophilic Archaeon Sulfolobus acidocaldarius. Frontiers in Bioengineering and Biotechnology, 3.
Vazquez-Armenta, F. J., Bernal-Mercado, A. T., Tapia-Rodriguez, M. R., Gonzalez-Aguilar, G. A., Lopez-Zavala, A. A., Martinez-Tellez, M. A., & Ayala-Zavala, J. F. (2018). Quercetin reduces adhesion and inhibits biofilm development by Listeria monocytogenes by reducing the amount of extracellular proteins. Food Control, 90, 266–273.
Memariani, H., Memariani, M., & Ghasemian, A. (2019). An overview on anti-biofilm properties of quercetin against bacterial pathogens. World Journal of Microbiology & Biotechnology, 35(9), 143.
Author information
Authors and Affiliations
Contributions
All authors contributed equally.
Corresponding author
Ethics declarations
Ethics Approval
Not applicable.
Consent to Participate
All authors have their consent to participate.
Consent to Publish
All authors have their consent to publish their work.
Competing Interests
The authors declare no competing interests.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Zhang, H., Li, S. & Cheng, Y. Antibiofilm Activity of Allicin and Quercetin in Treating Biofilm-Associated Orthopaedics Infection. Appl Biochem Biotechnol (2022). https://doi.org/10.1007/s12010-022-03845-4
Accepted:
Published:
DOI: https://doi.org/10.1007/s12010-022-03845-4