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Arthrospira platensis-Mediated Green Biosynthesis of Silver Nano-particles as Breast Cancer Controlling Agent: In Vitro and In Vivo Safety Approaches

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Abstract

Biogenic silver nanoparticles (bio-AgNPs) is one of the most fascinating nanomaterials used for several biomedical purposes. In the current study, we biosynthesized AgNPs (bio-AgNPs) using Arthrospira platensis (A-bio-AgNPs), Microcystis aeruginosa (M-bio-AgNPs), and Chlorella vulgaris (C-bio-AgNPs) active metabolites and evaluated their anticancer efficacy against breast cancer. The recovered bio-AgNPs were characterized using scanning and transmission electron microscopy (SEM and TEM). In addition, their safety profiles were monitored in vitro on PBMCs cells and in vivo on Albino mice. The obtained results indicated the safety usage of bio-AgNPs at concentrations of 0.1 mg/ml on PBMCs cells and 1.5 mg/ml on the Albino mice. The bio-AgNPs displayed dose-dependent cytotoxic effects against HepG-2, CaCO-2, and MCF-7 cell lines by inducing reactive oxygen species (ROS) and arresting the treated cells in G0/G1 and sub G0 phases. In addition, A-bio-AgNPs induced breast cancer cellular apoptosis by downregulating the expression of survivin, MMP7, TGF, and Bcl2 genes. Upon A-bio-AgNPs treatment, a significant reduction in tumor growth and prolonged survival rates were recorded in breast cancer BALB/c model. Furthermore, A-bio-AgNPs treatment significantly decreased the Ki-67 protein marker from 60% (in the untreated group) to 20% (in the treated group) and increased caspase-3 protein levels to 65% (in treated groups) comparing with 45% (in doxorubicin-treated groups).

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All data generated or analyzed during this study are included in this published article (and its supplementary information files).

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Authors and Affiliations

  1. Biopharmaceutical Products Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab City, 21934, Alexandria, Egypt

    Nehal M. EL-Deeb

  2. Botany and Microbiology Department, Faculty of Science, Tanta University, Tanta, Egypt

    Mai A. Abo-Eleneen, Omyma A. Awad & Atef M. Abo-Shady

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  1. Nehal M. EL-Deeb
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  2. Mai A. Abo-Eleneen
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  3. Omyma A. Awad
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Nehal: designing of the work, the acquisition, analysis, interpretation of data, drafted the work, and substantively revised.

Mai: Helped in the acquisition, analysis, interpretation of data, and drafted the work.

Omyma and Atef: Supervised, revised, and approved the submitted version.

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Correspondence to Nehal M. EL-Deeb.

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All in vivo studies were done according to the City of Scientific Research and Technological Applications, Egypt, guidelines. The Research Ethical Committee at the pharmaceutical industries center, Egypt, under international and institutional guidelines (REC-FPTU) approved all used experimental protocols.

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EL-Deeb, N.M., Abo-Eleneen, M.A., Awad, O.A. et al. Arthrospira platensis-Mediated Green Biosynthesis of Silver Nano-particles as Breast Cancer Controlling Agent: In Vitro and In Vivo Safety Approaches. Appl Biochem Biotechnol 194, 2183–2203 (2022). https://doi.org/10.1007/s12010-021-03751-1

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