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Synthesis of β-Glucan Nanoparticles from Red Algae–Derived β-Glucan for Potential Biomedical Applications

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Abstract

The present study highlights/demonstrates facile synthesis of β-Glucan nanoparticles (β-GluNPs) that can be used in the prevention of breast cancer and other infectious diseases. Moreover, this method is inexpensive and shows effectivity towards different biological applications. Further, the characterization of synthesized β-GluNPs was exclusively confirmed through UV–Vis spectroscopy, Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta potential, scanning electron microscopy (SEM), high resolution-transmission electron microscopy (HR-TEM), and X-ray powder diffraction (XRD) analysis. The synthesized β-GluNPs were further confirmed by FT-IR spectroscopy. The HR-TEM results demonstrated that the formation of polydispersed nanoparticles with a mean size of 20 ± 5 nm. The hydrostatic zeta potential was − 22.7 mV, which indicated their colloidal stability. The XRD pattern revealed the crystalline nature of the nanoparticles. Besides, β-GluNPs showed better antibacterial activity against the tested pathogens. The apoptosis and DNA fragmentation observed to be IC50 42.5 µg/ml of the β-GluNPs. The DNA fragmentation assay indicated the selective inhibition of the MCF-7 cell line by DNA damage. Hence, the study reports that the β-GluNPs have a potential to be used as a promising alternative drug against human breast cancer.

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Data Availability

The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

Parthasarathy Ramalingam (RP) received a research grant from DST-SERB, Govt of India, New Delhi, for the National Post-Doctoral fellowship (Ref. no. Dr. R. Parthasarathy Ramalingam PDF/2017/001184); Seetharaman Prabu Kumar (S.P.K.) received funding from Laboratory of Functional Molecules and Materials, School of Physics and Optoelectronic Engineering, Shandong University of Technology, Zibo 255000, China; H.C. Yashavantha Rao received UGC Kothari fellowship, New Delhi (No. F.4_2/2006 (BSR)/BL/17–18/0234); The authors also received support from confocal microscopy central facility, Department of Biochemistry, Division of Biological Sciences, Indian Institute of Science (IISc), Bangalore.

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Authors and Affiliations

  1. Department of Biochemistry, Indian Institute of Science, Bangalore, 560012, India

    Ramalingam Parthasarathy, H. C. Yashavantha Rao & Jayabaskaran Chelliah

  2. Laboratory of Functional Molecules and Materials, School of Physics and Optoelectronic Engineering, Shandong University of Technology, 255000, Xincun West Road 266, Zibo, China

    Seetharaman Prabu Kumar

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  1. Ramalingam Parthasarathy
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Contributions

Conceptualization of this research, design of experiments (R.P., S.P.K., and H.C.Y.R); Extraction of β-Glucan and synthesis, characterization of β-GluNPs (R.P., S.P.K., and H.C.Y.R); Performance of antibacterial and anticancer studies (R.P.); Drafting of the manuscript, (R.P. and S.P.K.); Writing–original draft preparation, supervision, proofreading, and manuscript design, (R.P., S.P.K., and J.C.); Funding acquisition (R.P., S.P.K., and J.C.). All authors have read and agreed to the published version of the manuscript.

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Correspondence to Ramalingam Parthasarathy.

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Parthasarathy, R., Kumar, S.P., Rao, H.C.Y. et al. Synthesis of β-Glucan Nanoparticles from Red Algae–Derived β-Glucan for Potential Biomedical Applications. Appl Biochem Biotechnol 193, 3983–3995 (2021). https://doi.org/10.1007/s12010-021-03674-x

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