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Combination of Adsorption and Cellulose Derivative Membrane Coating for Efficient Immobilization of Laccase

Applied Biochemistry and Biotechnology volume 193, pages 446–462 (2021)Cite this article

Abstract

Immobilization of enzyme based on combination of adsorption and cellulose derivative membrane coating was established in this work for the first time. Laccase, a commonly used enzyme in varied fields, was chosen as the model enzyme to demonstrate this method. After investigating operational conditions, the optimal process was obtained as follows: diatomite or HPD-417 as the adsorption carrier, 0.5% (w/v) methylcellulose (40,000~50,000) acetone solution as the coating solution, 0.75% (w/v) polyethylene glycol or maltose as the protective agent, and drying at 4 °C for 9 h. Under the optimal conditions, the residual activities of diatomite and HPD-417 immobilized laccase reached 99.33% and 94.15%, respectively. The study on properties showed that the immobilized laccases held high pH tolerance and thermal stability. The immobilized laccases were further applied to the indigo decolorization and 2, 4-dichlorophenol degradation. They showed high catalytic efficiency and could be reused for several batches. On the whole, the immobilization method developed in this work can effectively avoid the inactivation of laccase during immobilization and improve the stability of immobilized laccase. The laccase immobilized by this method shows obvious potential for environmental governance.

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Funding

This work was supported by the China Postdoctoral Science Foundation (2017T100373 and 2016M600417), the National Key R&D Program of China (2017YFD0600701), the Six Talent Peaks Project in Jiangsu Province (2015-JY-016), and the 333 Project of Jiangsu Province (BRA2017458).

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Affiliations

  1. Co-innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, China

    Rong Wu, Feixiang Liu, Qihui Dong & Erzheng Su

  2. Department of Food Science and Technology, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, China

    Rong Wu, Feixiang Liu, Qihui Dong, Yanyan Huang, Yibin Qiu, Yiyi Sun & Erzheng Su

  3. Co-Innovation Center for Efficient Processing and Utilization of Forest Products, Nanjing Forestry University, Nanjing, 210037, China

    Erzheng Su

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  1. Rong Wu
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  2. Feixiang Liu
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Correspondence to Erzheng Su.

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Wu, R., Liu, F., Dong, Q. et al. Combination of Adsorption and Cellulose Derivative Membrane Coating for Efficient Immobilization of Laccase. Appl Biochem Biotechnol 193, 446–462 (2021). https://doi.org/10.1007/s12010-020-03446-z

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  • DOI: https://doi.org/10.1007/s12010-020-03446-z

Keywords

  • Immobilization
  • Adsorption
  • Cellulose derivative coating
  • Laccase
  • Decolorization
  • Degradation