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Multilayered Nano-Entrapment of Lipase through Organic-Inorganic Hybrid Formation and the Application in Cost-Effective Biodiesel Production

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Abstract

Significant components of cost-effective medium for Magnusiomyces capitatus A4C extracellular lipase (ECL) production were optimized via a five-level factorial design. A simplistic, economical, and green approach was adopted for biomimetic mineralization to prepare multilayered nano-entrapped ECL, which were then applied as biocatalysts for the production of fatty acid methyl ester (FAME). The optimal ECL (0.8 mg protein/mL) and CuSO4∙5H2O (1.2 mM) showed the highest capacity for enzyme loading. The ECL-CuSO4-hybrid showed an 89.7% conversion of triacylglycerides into FAME via transesterification and a 98.7% conversion of oleic acid into FAME via esterification at 72 h. The ECL-CuSO4-hybrid gave 65% and 78.7% FAME production after 5 successive reuses via transesterification and esterification reactions, respectively. Therefore, these ECL-inorganic hybrid biocatalysts have high economical potential to be used for the production of biodiesel as the future petrodiesel replacement.

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Funding

This work was financially supported by the Higher Education Research Promotion and Thailand’s Education Hub for Southern Regions of ASEAN Countries Project Office of the Higher Education Commission under Contract No. THE-AC 024/2015. Besides, the research was funded by the Prince of Songkla University under Contract No. AGR6202081S. The second and third authors are supported by the Thailand Research Fund under Grant No. RTA6280014.

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Authors and Affiliations

  1. Molecular Biotechnology Laboratory, Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Khurshid Ahmed Baloch & Apichat Upaichit

  2. Biotechnology for Bioresource Utilization Laboratory, Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Khurshid Ahmed Baloch & Benjamas Cheirsilp

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  1. Khurshid Ahmed Baloch
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  2. Apichat Upaichit
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Correspondence to Apichat Upaichit.

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Highlights

• Cost-effective extracellular lipase production by Magnusiomyces capitatus A4C was optimized.

• Immobilization of extracellular lipase was performed by enzyme-inorganic mineralization.

• Application of lipase-inorganic hybrids for biodiesel production via transesterification and esterification.

• Achievement of 98% of biodiesel yield.

• Consecutive reuses of lipase-inorganic hybrids in different batches.

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Baloch, K.A., Upaichit, A. & Cheirsilp, B. Multilayered Nano-Entrapment of Lipase through Organic-Inorganic Hybrid Formation and the Application in Cost-Effective Biodiesel Production. Appl Biochem Biotechnol 193, 165–187 (2021). https://doi.org/10.1007/s12010-020-03404-9

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