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Reactive Green 5–Decorated Polyacrylamide/Chitosan Cryogel: An Affinity Matrix for Catalase

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Abstract

Acrylamide/chitosan-based cryogel was fabricated, and a triazine dye, Reactive Green 5, was attached to the cryogel by nucleophilic substitution to build a dye affinity support for adsorption of catalase enzyme. Characterization of cryogel was performed using FTIR, SEM, EDX, BET, and swelling test. Synthesized cryogel beared pores with ~ 200 μm in size and the surface area of 11.8 m2/g. Maximum catalase adsorption was (17.6 ± 0.29 mg/g) measured at pH 4.0 and 25 °C. The adsorption sites on the cryogel were saturated at 0.75 mg/mL enzyme concentration. Increased ionic strength caused a decrease in adsorption capacity. Desorption of catalase from cryogel was enabled using 0.5 M NaSCN solution. Consecutive adsorption experiments were carried out fifteen times to evaluate the reusability of the cryogel. Thermal, storage, and operational stabilities of immobilized catalase were higher than the free one. The data produced implicate that catalase-adsorbed dye-affinity cryogel may be used for H2O2 detection or removal when necessary.

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  • 03 August 2020

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Authors and Affiliations

  1. Chemistry Department, Faculty of Arts and Sciences, Aydın Adnan Menderes University, 09010, Aydın, Turkey

    Rukiye Yavaşer & Arife Alev Karagözler

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  1. Rukiye Yavaşer
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  2. Arife Alev Karagözler
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Correspondence to Rukiye Yavaşer.

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Highlights

• Reactive Green 5 bearing polyacrylamide/chitosan cryogel was synthesized by attaching the dye via nucleophilic substitution.

• Structural characterization of cryogel was carried out by FTIR, SEM, EDX, BET, and swelling test.

• Catalase enzyme was immobilized onto Reactive Green 5 bearing polyacrylamide/chitosan cryogel by adsorption, and conditions for maximum adsorption were determined.

• Kinetic properties of free and immobilized catalase enzymes were evaluated.

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Yavaşer, R., Karagözler, A.A. Reactive Green 5–Decorated Polyacrylamide/Chitosan Cryogel: An Affinity Matrix for Catalase. Appl Biochem Biotechnol 192, 1191–1206 (2020). https://doi.org/10.1007/s12010-020-03393-9

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  • DOI: https://doi.org/10.1007/s12010-020-03393-9

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