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Mead Production by Saccharomyces cerevisiae Safbrew T-58 and Saccharomyces bayanus (Premier Blanc and Premier Cuvée): Effect of Cowpea (Vigna unguiculata L. Walp) Extract Concentration

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Abstract

This work evaluated the effect of the cowpea (Vigna unguiculata L. Walp) extract concentration on mead production. Fermentations were carried out in 500-mL Erlenmeyer flasks containing 250 mL of honey wort (30 °Brix), supplemented with 1 g L−1 of ammonium sulfate and 0.1 g L−1 of magnesium chloride and the cowpea extract (5 and 30 g L−1), inoculated with 106 cells mL−1, and incubated at 30 °C for 240 h. Higher cell growth ((cells mL−1): 11.1 × 107, 11.3 × 107, and 19.6 × 107; substrate consumption (%): 86.0, 90.0, and 85.0) and ethanol production ((v v−1 %): 15.0, 15.5, and 14.1) for yeasts Safbrew T-58, Premier Blanc, and Premier Cuvée, respectively, were obtained with 30 g L−1 of bean extract. S. bayanus Premier Blanc had the best metabolic activity with lowest glycerol production (8.5 g L−1) and highest ethanol volumetric yields (0.51–1.52 h−1) after 48 h of fermentation.

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Acknowledgments

This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brazil (CAPES), Financial Code – 001, and Fundação de Amparo à Pesquisa do Estado de Bahia (FAPESB), Brazil. We thank the Graduate Program in Biotechnology (PPGBiotec) of the State University of Feira de Santana (UEFS).

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Authors and Affiliations

  1. Department of Technology, State University of Feira de Santana, Feira de Santana, BA, 44.036-900, Brazil

    Geiza Suzart Araújo, Mashely Pickman Gutiérrez, Kayque Frota Sampaio, Sílvia Maria Almeida de Souza & Ernesto Acosta Martínez

  2. Department of Biotechnology, Lorena Engineering School, University of São Paulo, Lorena, SP, 12602-810, Brazil

    Rita de Cássia Lacerda Brambilla Rodrigues

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  1. Geiza Suzart Araújo
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The presenting author of this manuscript in journal Applied Biochemistry and Biotechnology is Geiza Suzart Araújo.

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Araújo, G.S., Gutiérrez, M.P., Sampaio, K.F. et al. Mead Production by Saccharomyces cerevisiae Safbrew T-58 and Saccharomyces bayanus (Premier Blanc and Premier Cuvée): Effect of Cowpea (Vigna unguiculata L. Walp) Extract Concentration. Appl Biochem Biotechnol 191, 212–225 (2020). https://doi.org/10.1007/s12010-020-03267-0

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