Abstract
Batch methane fermentation was conducted using seed sludge collected from six methane fermentation facilities. Swine feces were centrifuged and autoclaved, followed by its use as a substrate for methanogenesis. This “swine feces supernatant medium” facilitates the cultivation of the microbes of the seed sludge, sampling of the digested liquid using a syringe, and subculturing of the digested liquid in a subsequent medium using a syringe. Through 15 subcultures, digested liquids with high and low methane production potential were obtained, which were named “H-DS” and “L-DS,” respectively. On the day 10 of cultivation, chemical oxygen demand (COD) of H-DS significantly decreased by 31% and that of L-DS did not differ significantly compared with that on the day 0 of cultivation. Acetic acid concentration of H-DS (1009 mg/L) was significantly lower than that of L-DS (2686 mg/L). These chemical characteristics indicate that organics decomposition in L-DS was not successful and suggest that H-DS has high relative abundance of bacteria decomposing organic matter and methanogen utilizing acetic acid compared with those in L-DS. Microbial community analysis revealed that Shannon index of H-DS was significantly higher than that of L-DS, and the relative abundance of acetogenic bacteria (e.g., Syntrophomonas) and acetic acid-utilizing methanogen (Methanosarcina) in H-DS was significantly higher than that in L-DS. Thus, the high methane production potential of H-DS might be attributable to the smooth flow from acetogenesis to methanogenesis step in the methane fermentation, compared with the case of L-DS.
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Acknowledgments
We thank Mitsui E&S Environment Engineering Co., Ltd., and Misaki Youton Corp. for providing the digested liquids, and Shoji Ookutsu (Kagoshima University) for providing the swine feces.
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This research was supported by Research grant for advanced research, United Graduate School of Agricultural Sciences, Kagoshima University.
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Nakamura, Y., Ishibashi, M., Kamitani, Y. et al. Microbial Community Analysis of Digested Liquids Exhibiting Different Methane Production Potential in Methane Fermentation of Swine Feces. Appl Biochem Biotechnol 191, 1140–1154 (2020). https://doi.org/10.1007/s12010-020-03228-7
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DOI: https://doi.org/10.1007/s12010-020-03228-7