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Immobilization of Thermoalkalophilic Lipase from Bacillus atrophaeus FSHM2 on Amine-Modified Graphene Oxide Nanostructures: Statistical Optimization and Its Application for Pentyl Valerate Synthesis

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Abstract

Synthesis of (3-aminopropyl) triethoxysilane (APTES)-functionalized graphene oxide (GO) nanosheets, statistical optimization of conditions for immobilization of Bacillus atrophaeus lipase (BaL) on as-synthesized support, and application of the immobilized BaL for esterification of valeric acid were carried out in this investigation. The optimum specific activity of the immobilized BaL (81.60 ± 0.28 U mg−1) was achieved at 3 mg mL−1 of GO-NH2, 50 mM of phosphate buffer, pH 7.0, 60 min sonication time, 100 mM glutaraldehyde, 25 U mL−1 of enzyme, and 8 h immobilization time at 4 °C. The immobilized BaL retained about 90% of its initial activity after 10 days of storage. Moreover, about 70% of the initial activity of the immobilized BaL was retained after 10 cycles of application. The results of esterification studies exhibited that maximum pentyl valerate synthesis using the free BaL (34.5%) and the immobilized BaL (92.7%) occurred in the organic solvent medium (xylene) after 48 h of incubation at 60 °C.

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Funding

This work was financially supported by Pharmaceutics Research Center, Kerman University of Medical Sciences, Kerman, Iran. Research reported in this publication was also supported by Elite Researcher Grant Committee under award number from the National Institutes for Medical Research Development (987592), Tehran, Iran.

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Authors and Affiliations

  1. Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

    Atefeh Ameri & Mojtaba Shakibaie

  2. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran

    Mojtaba Shakibaie & Hamid Forootanfar

  3. Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Mehdi Khoobi

  4. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Ali Faramarzi

  5. Department of Medicinal Chemistry, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran

    Alieh Ameri

  6. Pharmaceutical Sciences and Cosmetic Products Research Center, Kerman University of Medical Sciences, Kerman, Iran

    Hamid Forootanfar

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  1. Atefeh Ameri
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Ameri, A., Shakibaie, M., Khoobi, M. et al. Immobilization of Thermoalkalophilic Lipase from Bacillus atrophaeus FSHM2 on Amine-Modified Graphene Oxide Nanostructures: Statistical Optimization and Its Application for Pentyl Valerate Synthesis. Appl Biochem Biotechnol 191, 579–604 (2020). https://doi.org/10.1007/s12010-019-03180-1

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