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Enzymatic Activity of Urokinase Immobilized onto Cu2+-Chelated Cibacron Blue F3GA–Derived Poly (HEMA) Magnetic Nanoparticles

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Abstract

In this presented work, magnetic poly(2-hydroxyethyl methacrylate) (p (HEMA)) nanoparticles were synthesized by surfactant-free emulsion polymerization technique. Cibacron Blue F3GA was covalently attached to the magnetic p (HEMA) nanoparticles and Cu2+ ions were then chelated with dye molecules. Synthesized magnetic nanoparticles were spherical with the diameter of 80 nm and exhibited magnetic character. Incorporation rate of Cibacron Blue for magnetic nanoparticles was found to be 28.125-μmol/g polymer. Loaded amount of Cu2+ ions was calculated as 10.229-μmol/g polymer. These Cu2+-Cibacron Blue F3GA–derived magnetic p (HEMA) nanoparticles were used for urokinase adsorption under different conditions (i.e., pH, enzyme initial concentration, ionic strength, temperature). Maximum adsorption capacity was found to be 630.43-mg/g polymer, and it was observed that Langmuir adsorption isotherm was applicable in this adsorption process. The adsorbed urokinase was desorbed from the Cu2+-Cibacron Blue F3GA–derived magnetic p (HEMA) nanoparticles by using 1.0 M of NaCl with the desorption rate of 96%. It was also demonstrated that adsorption capacity did not change significantly after five adsorption/desorption cycles.

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Funding

This research has been supported by the Adnan Menderes University Research Fund under project number FEF-17002.

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Authors and Affiliations

  1. Faculty of Science and Arts, Chemistry Division, Adnan Menderes University, Aydın, Turkey

    Sinem Evli & Deniz Aktaş Uygun

  2. Nanotechnology Application and Research Center, Adnan Menderes University, Aydın, Turkey

    Deniz Aktaş Uygun

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  1. Sinem Evli
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  2. Deniz Aktaş Uygun
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Correspondence to Deniz Aktaş Uygun.

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Evli, S., Uygun, D.A. Enzymatic Activity of Urokinase Immobilized onto Cu2+-Chelated Cibacron Blue F3GA–Derived Poly (HEMA) Magnetic Nanoparticles. Appl Biochem Biotechnol 188, 194–207 (2019). https://doi.org/10.1007/s12010-018-2923-z

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