Skip to main content
SpringerLink
Log in

Preparation of Magnetic Nanoparticles and Their Use for Immobilization of C-Terminally Lysine-Tagged Bacillus sp. TS-23 α-Amylase

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The aim of this investigation was to synthesize the adipic acid-modified magnetic nanoparticles for the efficient immobilization of C-terminally lysine-tagged α-amylase (BACΔNC-Lys7) from thermophilic Bacillus sp. strain TS-23. The carboxylated magnetic nanoparticles were prepared by the simple co-precipitation of Fe3+/Fe2+ in aqueous medium and then subsequently modified with adipic acid. Transmission electron microscopy micrographs showed that the carboxylated magnetic nanoparticles remained discrete and had no significant change in size after the binding of BACΔNC-Lys7. Free enzyme was active in the temperature range of 45–70 °C and had an optimum of 60 °C, whereas the thermal stability of BACΔNC-Lys7 was improved as a result of immobilization. The immobilized BACΔNC-Lys7 could be recycled 20 times without a significant loss of the amylase activity and had a better stability during storage with respect to free enzyme. Taken together, the magnetic nanoparticles coated with this functional moiety can be a versatile platform for the effective manipulation of various kinds of engineered proteins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Polizzi, K. M., Bommarius, A. S., Broering, J. M., & Chaparro-Riggers, J. F. (2007). Current Opinion in Chemical Biology, 11, 220–225.

    Article  CAS  Google Scholar 

  2. Brady, D., & Jordaan, J. (2009). Biotechnology Letters, 31, 1639–1650.

    Article  CAS  Google Scholar 

  3. Katchalski-Katzir, E., Kraemer, D. M., & Eupergit, C. (2000). Journal of Molecular Catalysis B: Enzymatic, 10, 157–176.

    Article  CAS  Google Scholar 

  4. Straathof, A. J., Panke, S., & Schmid, A. (2002). Current Opinion in Biotechnology, 13, 548–556.

    Article  CAS  Google Scholar 

  5. Spahn, C., & Minteer, S. D. (2008). Recent Patents on Engineering, 2, 195–200.

    Article  CAS  Google Scholar 

  6. Tanyolac, D., Yürüksoy, B. I., & Özdural, A. R. (1998). Journal of Biochemical Engineering, 2, 179–186.

    Article  CAS  Google Scholar 

  7. Cong, L., Kaul, R., Dissing, U., & Mattiasson, B. (1995). Journal of Biotechnology, 42, 75–84.

    Article  CAS  Google Scholar 

  8. Ertan, F., Yagar, H., & Balkan, B. (2007). Preparative Biochemistry & Biotechnology, 37, 195–204.

    Article  CAS  Google Scholar 

  9. Shewale, S. D., & Pandit, A. B. (2007). Carbohydrate Research, 342, 997–1008.

    Article  CAS  Google Scholar 

  10. Raviyan, P., Tang, J., & Rasco, B. A. (2003). Journal of Agricultural and Food Chemistry, 51, 5462–5466.

    Article  CAS  Google Scholar 

  11. Dey, G., Nagpal, V., & Banerjee, R. (2002). Applied Biochemistry and Biotechnology, 102–103, 303–313.

    Article  Google Scholar 

  12. Sun, Y. M., Chen, J. P., & Chu, D. H. (1999). Journal of Biomedical Materials Research, 45, 125–132.

    Article  CAS  Google Scholar 

  13. Lim, Y. T., Lee, K. Y., Lee, K., & Chung, B. Y. (2006). Biochemical and Biophysical Research Communication, 344, 926–930.

    Article  CAS  Google Scholar 

  14. Jiang, Y., Guo, C., Xia, H., Mahmood, I., Liu, C., & Liu, H. (2009). Journal of Molecular Catalysis B: Enzymatic, 58, 103–109.

    Article  CAS  Google Scholar 

  15. Lo, H. F., Hu, H. Y., Hung, C. P., Chen, S. C., & Lin, L. L. (2009). Biocatalysis & Biotransformation, 27, 318–327.

    Article  CAS  Google Scholar 

  16. Li, D., Teoh, W. Y., Gooding, J. J., Selomulya, C., & Amal, R. (2010). Advanced Functional Materials, 20, 1767–1777.

    Article  CAS  Google Scholar 

  17. Berry, C. C. (2005). Journal of Material Chemistry, 15, 543–547.

    Article  CAS  Google Scholar 

  18. Mornet, S., Vasseur, S., Grasset, F., & Dugnet, E. (2004). Journal of Material Chemistry, 14, 2161–2175.

    Article  CAS  Google Scholar 

  19. Berry, C. C., & Curtis, A. S. G. (2003). Journal of Physics D: Applied Physics, 36, R198–R206.

    Article  CAS  Google Scholar 

  20. Osaka, T., Matsunaga, T., Nakanishi, T., Arakasi, A., Niwa, D., & Iida, H. (2006). Analytical and Bioanalytical Chemistry, 384, 593–600.

    Article  CAS  Google Scholar 

  21. Haun, J. B., Yoon, T. J., Lee, H., & Weissleder, R. (2010). Reviews: Nanomedicine and Nanobiotechnology, 2, 291–304.

    Article  CAS  Google Scholar 

  22. Lo, H. F., Lin, L. L., Li, C. C., Hsu, W. H., & Chang, C. T. (2001). Current Microbiology, 43, 170–175.

    Article  CAS  Google Scholar 

  23. Lin, L. L., Huang, C. C., & Lo, H. F. (2008). Process Biochemistry, 43, 559–565.

    Article  CAS  Google Scholar 

  24. Chi, M. C., Chen, Y. H., Wu, T. J., Lo, H. F., & Lin, L. L. (2010). Journal of Bioscience and Bioengineering, 109, 531–538.

    Article  CAS  Google Scholar 

  25. Namdeo, M., & Baipai, S. K. (2009). Journal of Molecular Catalysis B: Enzymatic, 59, 134–139.

    Article  CAS  Google Scholar 

  26. Khalafalla, S. E., & Reimers, G. W. (1980). IEEE Transactions on Magnetics, 16, 178–183.

    Article  Google Scholar 

  27. Miller, G. L. (1959). Analytical Chemistry, 31, 426–428.

    Article  CAS  Google Scholar 

  28. Terpe, K. (2003). Applied Microbiology and Biotechnology, 60, 523–533.

    CAS  Google Scholar 

  29. Wu, C. W., Lee, J. G., & Lee, W. C. (1998). Biotechnology and Applied Biochemistry, 27, 225–230.

    CAS  Google Scholar 

  30. Ikediobi, C. O., Stevens, M., & Latinwo, L. (1998). Process Biochemistry, 33, 491–494.

    Article  CAS  Google Scholar 

  31. Zhou, J., Wu, W., Caruntu, D., Yu, M. H., Martin, A., Chen, J. F., et al. (2007). Journal of Physical Chemistry C, 111, 17473–17477.

    Article  CAS  Google Scholar 

  32. Arica, M. Y., Alaeddinoglu, N. G., & Hasirei, V. (1998). Enzyme and Microbial Technology, 22, 152–157.

    Article  CAS  Google Scholar 

  33. Oh, J. T., & Kim, J. H. (2000). Enzyme and Microbial Technology, 27, 356–361.

    Article  CAS  Google Scholar 

  34. Li, G. Y., Huang, K. L., Jiang, Y. R., Yang, D. L., & Ding, P. (2008). International Journal of Biological Macromolecules, 42, 405–412.

    Article  CAS  Google Scholar 

  35. Tsang, S. C., Yu, C. H., Gao, X., & Tam, K. (2006). Journal of Physical Chemistry B, 110, 16914–16922.

    Article  CAS  Google Scholar 

  36. Li, J., Wang, J., Gavalas, V. G., Atwood, D. A., & Bachas, L. G. (2003). Nano Letters, 3, 55–58.

    Article  CAS  Google Scholar 

  37. Takahashi, H., Li, B., Sasaki, T., Miyazaki, C., Kajino, T., & Inagaki, S. (2000). Chemical Materials, 12, 3301–3305.

    Article  CAS  Google Scholar 

  38. Akgöl, S., Yalcinkaya, Y., Bayramoglu, A., Denizli, A., & Arica, M. Y. (2002). Process Biochemistry, 38, 675–683.

    Article  Google Scholar 

  39. Martino, A., Pifferi, P. G., & Spagna, G. (1996). Process Biochemistry, 31, 243–248.

    Article  Google Scholar 

  40. Trzmiel, T., Fortak, M., & Galas, E. (1995). Acta Biotechnology, 15, 123–129.

    Article  CAS  Google Scholar 

  41. Jiang, D. S., Long, S. Y., Huang, J., Xiao, H. Y., & Zhou, J. Y. (2005). Biochemical Engineering Journal, 25, 15–23.

    Article  Google Scholar 

  42. Cetinus, S. A., & Öztop, H. N. (2003). Enzyme and Microbial Technology, 32, 889–894.

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by the research grant (NSC 97-2628-B-415-001-MY3) from the National Science Council of Taiwan.

Author information

Authors and Affiliations

  1. Department of Applied Chemistry, National Chiayi University, 300 Syuefu Road, Chiayi County, 60004, Taiwan

    Yan-Hung Chen, Meng-Chun Chi, Jui-Chang Chen & Long-Liu Lin

  2. Department of Life Sciences and Institute of Molecular Biology, National Chung Cheng University, 168 University Road, Minshiung Township, Chiayi County, 62102, Taiwan

    Tzu-Fan Wang

Authors
  1. Yan-Hung Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Meng-Chun Chi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tzu-Fan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jui-Chang Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Long-Liu Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Long-Liu Lin.

Additional information

Y.-H. Chen and M.-C. Chi made equal contribution to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, YH., Chi, MC., Wang, TF. et al. Preparation of Magnetic Nanoparticles and Their Use for Immobilization of C-Terminally Lysine-Tagged Bacillus sp. TS-23 α-Amylase. Appl Biochem Biotechnol 166, 1711–1722 (2012). https://doi.org/10.1007/s12010-012-9575-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-012-9575-1

Keywords

Search

Navigation