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Inhibitors Compounds on Sugarcane Bagasse Saccharification: Effects of Pretreatment Methods and Alternatives to Decrease Inhibition

Applied Biochemistry and Biotechnology volume 188, pages 29–42 (2019)Cite this article

Abstract

Considering bioethanol production, extensive research has been performed to decrease inhibitors produced during pretreatments, to diminish energy input, and to decrease costs. In this study, sugarcane bagasse was pretreated with NaOH, H2SO4, and water. The higher concentration of phenols, 3.3 g/L, was observed in biomass liquid fraction after alkaline pretreatment. Acid pretreatment was responsible to release considerable acetic acid concentration, 2.3 g/L, while water-based pretreatment was the only to release formic acid, 0.02 g/L. Furans derivatives were not detected in liquid fractions regardless of pretreatment. Furthermore, washing step removed most of the phenols from pretreated sugarcane bagasse. Saccharification of alkali-pretreated biomass plus polyethylene glycol (PEG) at 0.4% (w/v) enhanced 8 and 26% the glucose and the xylose release, respectively, while polyvinylpyrrolidone (PVP) also at 0.4% (w/v) increased the release by 10 and 31% of these sugars, respectively, even without washing and filtration steps. Moreover, these polymers cause above 50% activation of endoglucanase and xylanase activities which are crucial for biomass hydrolysis.

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Acknowledgments

The authors would like to thank the CNPq for the scholarship granted to the first author. This research was also supported by the Brazilian institutions FAPEMIG and CAPES.

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Affiliations

  1. Department of Biochemistry and Molecular Biology, BIOAGRO, Federal University of Viçosa, Viçosa, MG, 36570-000, Brazil

    Rafaela I. S. Ladeira-Ázar, Túlio Morgan & Valéria M. Guimarães

  2. Department of Food and Nutrition, Federal University of Mato Grosso, Cuiabá, MT, 78060-900, Brazil

    Gabriela Piccolo Maitan-Alfenas

Authors
  1. Rafaela I. S. Ladeira-Ázar
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  2. Túlio Morgan
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  3. Gabriela Piccolo Maitan-Alfenas
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Correspondence to Gabriela Piccolo Maitan-Alfenas.

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Ladeira-Ázar, R.I.S., Morgan, T., Maitan-Alfenas, G.P. et al. Inhibitors Compounds on Sugarcane Bagasse Saccharification: Effects of Pretreatment Methods and Alternatives to Decrease Inhibition. Appl Biochem Biotechnol 188, 29–42 (2019). https://doi.org/10.1007/s12010-018-2900-6

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  • DOI: https://doi.org/10.1007/s12010-018-2900-6

Keywords

  • Sugarcane bagasse pretreatment
  • Inhibitors
  • Enzymes
  • Polyethylene glycol
  • Polyvinylpyrrolidone