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Compositional Shift in Fatty Acid Profiles of Lipids Obtained from Oleaginous Yeasts upon the Addition of Essential Oil from Citrus sinensis L.

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Abstract

Tailoring lipids from oleaginous yeasts to contain specific types of fatty acid is of considerable interest to food, fuel, and pharmaceutical industries. In this study, the essential oil obtained from Citrus sinesus L. has been used to alter the fatty acid composition of two common oleaginous yeasts, Rhodosporidium toruloides and Cryptococcus curvatus. With increasing levels of essential oil in the medium, the metabolic flux of the fatty acid biosynthesis pathway shifted towards saturated fatty acid production. Essential oil reduced the activities of elongase and ∆9 desaturase. This made the lipid obtained from both these yeasts rich in saturated fatty acids. At certain specific concentrations of the essential oil in the medium, the lipid obtained from R. toruloides and C. curvatus cultures was similar to mahuwa butter and palm oil, respectively. Limonene is the major constituents of orange essential oil. Its effect on one of the oleaginous yeasts, R. toruloides, was also studied separately. Effects similar to orange essential oil were obtained with limonene. Thus, we can conclude that limonene in orange essential oil brings about compositional change of microbial lipid produced in this organism.

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Acknowledgements

We would like to express our gratefulness to all the members of Lakehead University Centre for Analytical Services Environmental Laboratory and Lakehead University Instrumentation Laboratory (LUCAS) who helped us carry out certain analysis required as a part of this work. The authors would also like to acknowledge the support of Canada Foundation of Innovation (CFI) and Natural Sciences and Engineering Research Council of Canada (NSERC) for their financial support to undertake the PhD work in this area.

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Authors and Affiliations

  1. Department of Biotechnology, Lakehead University, Thunder Bay, Ontario, Canada

    Bijaya K. Uprety & Sudip K. Rakshit

  2. Biorefining Research Institute, Lakehead University, Thunder Bay, Ontario, Canada

    Bijaya K. Uprety & Sudip K. Rakshit

  3. Department of Chemical Engineering, Lakehead University, Thunder Bay, Ontario, Canada

    Sudip K. Rakshit

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Correspondence to Sudip K. Rakshit.

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Uprety, B.K., Rakshit, S.K. Compositional Shift in Fatty Acid Profiles of Lipids Obtained from Oleaginous Yeasts upon the Addition of Essential Oil from Citrus sinensis L.. Appl Biochem Biotechnol 183, 1158–1172 (2017). https://doi.org/10.1007/s12010-017-2490-8

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