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New Insight into Sugarcane Industry Waste Utilization (Press Mud) for Cleaner Biobutanol Production by Using C. acetobutylicum NRRL B-527

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Abstract

In the present study, press mud, a sugar industry waste, was explored for biobutanol production to strengthen agricultural economy. The fermentative production of biobutanol was investigated via series of steps, viz. characterization, drying, acid hydrolysis, detoxification, and fermentation. Press mud contains an adequate amount of cellulose (22.3%) and hemicellulose (21.67%) on dry basis, and hence, it can be utilized for further acetone-butanol-ethanol (ABE) production. Drying experiments were conducted in the temperature range of 60–120 °C to circumvent microbial spoilage and enhance storability of press mud. Furthermore, acidic pretreatment variables, viz. sulfuric acid concentration, solid to liquid ratio, and time, were optimized using response surface methodology. The corresponding values were found to be 1.5% (v/v), 1:5 g/mL, and 15 min, respectively. In addition, detoxification studies were also conducted using activated charcoal, which removed almost 93–97% phenolics and around 98% furans, which are toxic to microorganisms during fermentation. Finally, the batch fermentation of detoxified press mud slurry (the sample dried at 100 °C and pretreated) using Clostridium acetobutylicum NRRL B-527 resulted in a higher butanol production of 4.43 g/L with a total ABE of 6.69 g/L.

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Acknowledgements

The authors gratefully acknowledge the Department of Science and Technology (DST) of the Ministry of Science and Technology, Government of India, for providing financial support under the scheme of the DST INSPIRE Faculty Award (IFA 13-ENG-68/July 28, 2014) during the course of this investigation. The authors are also thankful to Radhika Malkar and Manoj Kamble from the Institute of Chemical Technology, Mumbai, for their help with SEM analysis.

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  1. Bharati Vidyapeeth Deemed University College of Engineering, Pune, 411043, India

    Pranhita R. Nimbalkar, Manisha A. Khedkar, Prakash V. Chavan & Sandip B. Bankar

  2. Chemical Engineering and Process Development Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, 411008, India

    Shashank G. Gaikwad

  3. Department of Bioproducts and Biosystems, Aalto University School of Chemical Engineering, P.O. Box 16100, FI-00076, Aalto, Finland

    Sandip B. Bankar

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  1. Pranhita R. Nimbalkar
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Nimbalkar, P.R., Khedkar, M.A., Gaikwad, S.G. et al. New Insight into Sugarcane Industry Waste Utilization (Press Mud) for Cleaner Biobutanol Production by Using C. acetobutylicum NRRL B-527. Appl Biochem Biotechnol 183, 1008–1025 (2017). https://doi.org/10.1007/s12010-017-2479-3

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