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Improved Performance of Lipase Immobilized on Tannic Acid-Templated Mesoporous Silica Nanoparticles

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Abstract

Mesoporous silica nanoparticles were synthesized by using tannic acid as a pore-forming agent, which is an environmentally friendly, cheap, and non-surfactant template. SEM and TEM images indicated that the tannic acid-templated mesoporous silica nanoparticles (TA-MSNs) are monodisperse spherical-like particles with an average diameter of 195 ± 16 nm. The Brunauer–Emmett–Teller (BET) results showed that the TA-MSNs had a relatively high surface area (447 m2/g) and large pore volume (0.91 cm3/g), and the mean pore size was ca. 10.1 nm. Burkholderia cepacia lipase was immobilized on the TA-MSNs by physical adsorption for the first time, and the properties of immobilized lipase (BCL@TA-MSNs) were investigated. The BCL@TA-MSNs exhibited satisfactory thermal stability; strong tolerance to organic solvents such as methanol, ethanol, isooctane, n-hexane, and tetrahydrofuran; and high operational reusability when BCL@TA-MSNs were applied in esterification and transesterification reactions. After recycling 15 times in the transesterification reaction for biodiesel production, over 85 % of biodiesel yield can be maintained. With these desired characteristics, the TA-MSNs may provide excellent candidates for enzyme immobilization.

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Acknowledgments

This work was supported by the National Nature Science Foundation of China (Nos. 21276060, 21276062, and 21306039), the Natural Science Foundation of Tianjin (13JCYBJC18500), the Science and Technology Research Key Project of Higher School in Hebei Province (YQ2013025), the Natural Science Foundation of Hebei Province (B2015202082), Project of Science and Technology of Hebei Province (13273607 and 13274314), and Tianjin City High School Science & Technology Fund Planning Project (20140513).

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  1. School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, 300130, China

    Yanjun Jiang, Wenya Sun, Liya Zhou, Li Ma, Ying He & Jing Gao

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Correspondence to Jing Gao.

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Jiang, Y., Sun, W., Zhou, L. et al. Improved Performance of Lipase Immobilized on Tannic Acid-Templated Mesoporous Silica Nanoparticles. Appl Biochem Biotechnol 179, 1155–1169 (2016). https://doi.org/10.1007/s12010-016-2056-1

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