Skip to main content
SpringerLink
Log in

Immobilization of lipase onto aminopropyl-functionalized MSU-H type mesoporous silica and esterification

  • Materials (Organic, Inorganic, Electronic, Thin Films)
  • Published:
Korean Journal of Chemical Engineering Aims and scope Submit manuscript

Abstract

Candida rugosa lipase (CRL) was immobilized on an aminopropyl-functionalized MSU-H type mesoporous silica (AFMS) through physical adsorption and a covalent cross-linking. It was evaluated as a class of biocatalysts in the esterification of conjugated linoleic acid (CLA) isomers with ethanol. AFMS materials with varied content of aminopropyl were prepared by a simple co-condensation at near neutral pH condition. Introduction of aminopropyl chains and CRL molecules onto the AFMS supports was confirmed by Fourier transform infrared (FT-IR) spectra. CRL was immobilized on the AFMS through electrostatic and covalent interactions. The covalently cross-linked CRL gave a loading amount of 34.3mg CRL/g-support and a hydrolytic activity of 2471.5U/g-catalyst. It exhibited high operational stability and remained 23.9-27.5% of total esterification in 32 h consecutive four runs in the esterification of CLA with ethanol. Moreover, the immobilized CRLs catalyzed 2.8-3.8 times of esterification of cis-(c)9, trans-(t)11-CLA faster than that of t10, c12-CLA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. E. Hernández-Martín and C. Otero, J. Agric. Food Chem., 57, 701 (2009).

    Article  Google Scholar 

  2. I.A. Hutchinson, A. A. Hennessy, R. J. Dewhurst, A. C.O. Evans, P. Lonergan and S. T. Butler, J. Dairy Sci., 95, 2442 (2012).

    Article  CAS  Google Scholar 

  3. T.Y. Yen, B. Stephen Inbaraj, J. T. Chien and B. H. Chen, Anal. Biochem., 400, 130 (2010).

    Article  CAS  Google Scholar 

  4. C. Rodriguez-Sallaberry, C. Caldari-Torres, E. S. Greene and L. Badinga, J. Dairy Sci., 89, 3826 (2006).

    Article  CAS  Google Scholar 

  5. S. J. Park, C.W. Park, S. J. Kim, J.K. Kim, Y.R. Kim, K.A. Park, J. O. Kim and Y. L. Ha, J. Agric. Food Chem., 50, 989 (2002).

    Article  CAS  Google Scholar 

  6. H. G. Park, H.T. Cho, M. C. Song, S.B. Kim, E. G. Kwon, N. J. Choi and Y. J. Kim, J. Agric. Food Chem., 60, 3204 (2012).

    Article  CAS  Google Scholar 

  7. E. Ostrowska, M. Muralitharan, R. F. Cross, D. E. Bauman and F.R. Dunshea, J. Nutr., 129, 2037 (1999).

    CAS  Google Scholar 

  8. A. Thierry, M.B. Maillard, R. Richoux and S. Lortal, J. Agric. Food Chem., 54, 6819 (2006).

    Article  CAS  Google Scholar 

  9. Y. H. Wang, X. F. Li, Y. X. Liang, B. Yang and S. H. Zhang, J. Mol. Catal. B-Enzym., 46, 20 (2007).

    Article  Google Scholar 

  10. U. H. Zaidan, M. B. Abdul Rahman, M. Basri, S. S. Othman, R. N. Z. R. Abdul Rahman and A. B. Salleh, Appl. Clay Sci., 47, 276 (2010).

    Article  CAS  Google Scholar 

  11. V. Dandavate, H. Keharia and D. Madamwar, Biocatal. Biotransform., 29, 37 (2011).

    Article  CAS  Google Scholar 

  12. S. Gao, Y. J. Wang, X. Diao, G. S. Luo and Y. Y. Dai, Bioresour. Technol., 101, 3830 (2010).

    Article  CAS  Google Scholar 

  13. C. Ispas, I. Sokolov and S. Andreescu, Anal. Bioanal. Chem., 393, 543 (2009).

    Article  CAS  Google Scholar 

  14. Y. Li, W. Wang and P. F. Han, Korean J. Chem. Eng., 31, 98 (2014).

    Article  CAS  Google Scholar 

  15. S. S. Kim, A. Karkamkar, T. J. Pinnavaia, M. Kruk and M. Jaroniec, J. Phys. Chem. B, 105, 7663 (2001).

    Article  CAS  Google Scholar 

  16. M. M. Bradford, Anal. Biochem., 72, 248 (1976).

    Article  CAS  Google Scholar 

  17. T. Vorderwülbecke, K. Kieslich and H. Erdmann, Enzyme Microb. Technol., 14, 631 (1992).

    Article  Google Scholar 

  18. H. S. Sohn, S. S. Chung and J. S. Rhee, Biotechnol. Lett., 9, 117 (1987).

    Article  CAS  Google Scholar 

  19. L. Bergman, J. Rosenholm, A.B. öst, A. Duchanoy, P. Kankaanpää, J. Heino and M. Lindén, J. Nanomater., 2008, 1 (2008).

    Google Scholar 

  20. J. M. Rosenholm and M. Lindén, Chem. Mater., 19, 5023 (2007).

    Article  CAS  Google Scholar 

  21. B. Dragoi and E. Dumitriu, Acta Chimica Slovenica, 55, 277 (2008).

    CAS  Google Scholar 

  22. W. H. Yu, M. Fang, D. S. Tong, P. Shao, T. N. Xu and C. H. Zhou, Biochem. Eng. J., 70, 97 (2013).

    Article  CAS  Google Scholar 

  23. X. Meng, G. Xu, Q. L. Zhou, J. P. Wu and L.R. Yang, Food Chem., 143, 319 (2014).

    Article  CAS  Google Scholar 

  24. Y. Jiang, L. Shi, Y. Huang, J. Gao, X. Zhang and L. Zhou, ACS Appl. Mater. Interfaces, 6, 2622 (2014).

    Article  CAS  Google Scholar 

  25. W. Chouyyok, J. Panpranot, C. Thanachayanant and S. Prichanont, J. Mol. Catal. B-Enzym., 56, 246 (2009).

    Article  CAS  Google Scholar 

  26. A. S. M. Chong and X. S. Zhao, J. Phys. Chem. B, 107, 12650 (2003).

    Article  CAS  Google Scholar 

  27. Sujandi, E. A. Prasetyanto and S. E. Park, Appl. Catal. A-Gen., 350, 244 (2008).

    Article  Google Scholar 

  28. A. Vinu, M. Miyahara and K. Ariga, J. Phys. Chem. B, 109, 6436 (2005).

    Article  CAS  Google Scholar 

  29. S. J. Gregg and K. S.W. Sing, Adsorption, surface area and porosity, Academic Press, London (1982).

    Google Scholar 

  30. A. Vinu, V. Murugesan, O. Tangermann and M. Hartman, Chem. Mater., 16, 3056 (2004).

    Article  CAS  Google Scholar 

  31. X. Zhang, R. F. Guan, D.Q. Wu and K.Y. Chan, J. Mol. Catal. BEnzym., 33, 43 (2005).

    Article  Google Scholar 

  32. Y.Q. Xu, G.W. Zhou, C.C. Wu, T.D. Li and H.B. Song, Solid State Sci., 13, 867 (2011).

    Article  CAS  Google Scholar 

  33. K. Kannan and R.V. Jasra, J. Mol. Catal. B-Enzym., 56, 34 (2009).

    Article  CAS  Google Scholar 

  34. A.Z. Abdullah, N. S. Sulaiman and A. H. Kamaruddin, Biochem. Eng. J., 44, 263 (2009).

    Article  CAS  Google Scholar 

  35. X. Zhang, F. Zhang and K.Y. Chan, Scripta Mater., 51, 343 (2014).

    Article  Google Scholar 

  36. C. H. Lee, T. S. Lin and C. Y. Mou, Nano Today, 4, 165 (2009).

    Article  CAS  Google Scholar 

  37. C. F. Wang, G.W. Zhou, Y. J. Li, N. Lu, H. B. Song and L. Zhang, Colloids Surf., A: Physicochem. Eng. Aspects, 406, 75 (2012).

    Article  CAS  Google Scholar 

  38. N. Neerupma, R. Singh and J. Kaur, J. Biotechnol., 9, 559 (2006).

    Google Scholar 

  39. A. S. M. Chong and X. S. Zhao, Catal. Today, 93–95, 293 (2004).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Zhijiang College, Zhejiang University of Technology, Hangzhou, 310024, P. R. China

    Wei Hua Yu

  2. Research Group for Advanced Materials & Sustainable Catalysis (AMSC), College of Chemical Engineering, Zhejiang University of Technology, Hangzhou, 310014, P. R. China

    Han Bin Zhao, Dong Shen Tong & Chun Hui Zhou

  3. College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310014, P. R. China

    Ping Shao

Authors
  1. Wei Hua Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Han Bin Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dong Shen Tong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chun Hui Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ping Shao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Wei Hua Yu or Chun Hui Zhou.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yu, W.H., Zhao, H.B., Tong, D.S. et al. Immobilization of lipase onto aminopropyl-functionalized MSU-H type mesoporous silica and esterification. Korean J. Chem. Eng. 32, 1694–1700 (2015). https://doi.org/10.1007/s11814-014-0391-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11814-014-0391-x

Keywords

Search

Navigation