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Molecular Approach Coupled with Biochemical Attributes to Elucidate the Presence of DYMV in Leaf Samples of Lablab purpureus. L Genotypes

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Abstract

A laboratory study was delineated to ascertain the impact and the extent of Dolichos yellow mosaic virus (DYMV) on biochemical constituents and various enzyme levels in the leaves of hyacinth bean. DYMV-infected leaves of all the genotypes used in the study revealed significant and consistent changes in activities of CAT, APX, PPO, DHAR, and MDHAR paralleled with a compelling hike in proline levels. Unlike that in non-infected leaves of the genotypes VRSEM-301 and VRSEM-749, VRSEM-894 and VRSEM-855, the enzyme level did not alter much which extended equally with increased phenolics, suggesting a well-coordinated generation of free radicals thereby suppressing oxidative stress in the latter. The genotypes were also evaluated at molecular level for elucidating the presence of the virus by using five sets of primer pairs. Two primers viz., DAC1 and DAC2 witnessed the presence of the virus in both non-infected and infected leaves. The difference in the appearance and/or disappearance of bands according to non-infected to infect reverberates the variation between genotypes in defense against infection.

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Abbreviations

DYMV:

Dolichos yellow mosaic virus

DHAR:

Dehydroascorbate reductase

MDHAR:

Monodehydroascorbate reductase

APX:

Ascorbate peroxidase

PPO:

Polyphenol oxidase

ROS:

Reactive oxygen species

CAT:

Catalase

Chl a:

Chlorophyll a

Chl b:

Chlorophyll b

CAR:

Carotenoid

Pro:

Proline

DAS:

Days after sowing

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Acknowledgment

The authors are thankful to the Director, Indian Institute of Vegetable Research, Varanasi for providing all of the necessary funds and facilities for conducting the research.

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Authors and Affiliations

  1. Indian Institute of Vegetable Research, Post Box-01, P.O.-Jakhani (Shahnshahpur), Varanasi, 221305, Uttar Pradesh, India

    Nagendra Rai, Krishna Kumar Rai, V. Venkataravanappa & Sujoy Saha

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  1. Nagendra Rai
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  2. Krishna Kumar Rai
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  3. V. Venkataravanappa
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  4. Sujoy Saha
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Correspondence to Nagendra Rai.

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Contribution

Dr Nagendra Rai—Experiment design and manuscript correction, Mr. Krishna Kumar Rai—performed all biochemical, molecular experiment, and preparation of manuscript, Dr V. Venkataravanappa—recorded the meteorological data and DYMV incidence data, Dr Sujoy Saha—manuscript correction.

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Rai, N., Rai, K.K., Venkataravanappa, V. et al. Molecular Approach Coupled with Biochemical Attributes to Elucidate the Presence of DYMV in Leaf Samples of Lablab purpureus. L Genotypes. Appl Biochem Biotechnol 178, 876–890 (2016). https://doi.org/10.1007/s12010-015-1915-5

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