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Batch and Pulsed Fed-Batch Cultures of Aspergillus flavipes FP-500 Growing on Lemon Peel at Stirred Tank Reactor

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Abstract

Aspergillus flavipes FP-500 grew up on submerged cultures using lemon peel as the only carbon source, developing several batch and pulsed fed-batch trials on a stirred tank reactor. The effect of carbon source concentration, reducing sugar presence and initial pH on exopectinase and endopectinase production, was analyzed on batch cultures. From this, we observed that the highest substrate concentration favored biomass (X max) but had not influence on the corresponding specific production (q p) of both pectinases; the most acid condition provoked higher endopectinase-specific productions but had not a significant effect on those corresponding to exopectinases; and reducing sugar concentrations higher than 1.5 g/L retarded pectinase production. On the other hand, by employing the pulsed fed-batch operation mode, we observed a prolonged growth phase, and an increase of about twofold on endopectinase production without a significant raise on biomass concentration. So, pulsed fed-batch seems to be a good alternative for obtaining higher endopectinase titers by using high lemon peel quantities without having mixing and repression problems to the system. The usefulness of unstructured kinetic models for explaining, under a theoretic level, the behavior of the fungus along the batch culture with regard to pectinase production was evident.

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Abbreviations

μ max :

The specific growth rate of the microorganism. This parameter is calculated by the logistic model, by the corresponding algorithm, 1/h

α:

The Luedeking–Piret parameter that indicates the enzyme production that is growth-related, or during the growth phase, U/gbiomass

β:

The Luedeking–Piret parameter that indicates the enzyme production that is growth independent, occurs during the maintenance phase, U/h gbiomass

q p :

The specific enzyme production, Uenzyme/gbiomass

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Acknowledgments

VEWM thanks COMECYT and EGG thanks CONACYT for the scholarship. This work was developed using funds from projects COMECYT EDOMEX-CO1-78011 and DGAPA IN219813.

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Authors and Affiliations

  1. Chemical and Biochemical Engineering Division, Technologic Institute for Higher Studies of Ecatepec, Av. Tecnológico esq. Av. Carlos Hank González, Col. Valle de Anáhuac, 55210, Ecatepec de Morelos, DF, Mexico

    V. E. Wolf-Márquez, E. García-García, M. García-Rivero & M. A. Martínez Trujillo

  2. Faculty of Chemistry, Department of Food Science and Biotechnology, National University of Mexico, Ciudad Universitaria, Conjunto E Química, 04510, Mexico, DF, Mexico

    G. Aguilar-Osorio

Authors
  1. V. E. Wolf-Márquez
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  2. E. García-García
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  3. M. García-Rivero
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  4. G. Aguilar-Osorio
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  5. M. A. Martínez Trujillo
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Correspondence to M. A. Martínez Trujillo.

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Wolf-Márquez, V.E., García-García, E., García-Rivero, M. et al. Batch and Pulsed Fed-Batch Cultures of Aspergillus flavipes FP-500 Growing on Lemon Peel at Stirred Tank Reactor. Appl Biochem Biotechnol 177, 1201–1215 (2015). https://doi.org/10.1007/s12010-015-1807-8

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  • DOI: https://doi.org/10.1007/s12010-015-1807-8

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