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Semi-rational Directed Evolution of Monoamine Oxidase for Kinetic Resolution of rac-Mexiletine

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Abstract

Semi-rational directed evolution was applied to the D5 variant of monoamine oxidase from Aspergillus niger (MAO-N-D5) with the aim of deriving the more desirable (R)-mexiletine through the kinetic resolution of mexiletine enantiomers. Although MAO-N-D5 shows no activity towards rac-mexiletine, theoretical molecular docking studies revealed the potential binding conformations of both mexiletine enantiomers and MAO-N-D5. The key factors affecting the catalytic activity and specificity were identified. Based on the docking results, six residues in the binding pocket and along the binding pathway were selected as key sites for saturation mutagenesis of MAO-N-D5. Through several rounds of screening and combinatorial experiments, two active MAO variants with high enantioselectivities towards (S)-mexiletine evolved, namely A-1 (F210V/L213C, E = 101) and AC-1 (F210V/I367T, E = 69). Molecular simulation experiments indicated that the introduced activity of these variants may be due to the reduced steric hindrance in the binding pocket of the relatively small-sized amino acid residues, a synergetic effect of the entrance residue mutation, and the formation of a new disulfide bond.

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Acknowledgments

This work was supported by the Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions (CIT&TCD20130324) and National Natural Science Foundation of China (Grant No. 31100584).

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Authors and Affiliations

  1. Beijing Key Laboratory for Dairy Cow Nutrition, Beijing University of Agriculture, Changping District, 102206, Beijing, China

    Linshu Jiang

  2. Laboratory of Biocatalysis, College of Life & Environmental Sciences, Hangzhou Normal University, Hangzhou, 311121, Zhejiang, China

    Zhenming Chen, Yuanhui Ma, Mengyan He, Shuo Zhou & Dunyue Lai

  3. Institute of Aging Research, School of Medicine, Hangzhou Normal University, Hangzhou, 311121, Zhejiang, China

    Zhiguo Wang

  4. Zhejiang Apeloa Tospo Pharmaceutical Co., Ltd., 519 Jiangnan Road, Hengdian Town, Dongyang, 322118, Zhejiang, China

    Hongyang Ren

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  1. Zhenming Chen
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  2. Yuanhui Ma
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Correspondence to Zhiguo Wang or Linshu Jiang.

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Chen, Z., Ma, Y., He, M. et al. Semi-rational Directed Evolution of Monoamine Oxidase for Kinetic Resolution of rac-Mexiletine. Appl Biochem Biotechnol 176, 2267–2278 (2015). https://doi.org/10.1007/s12010-015-1716-x

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