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Integration of Quercetin-Iron Complexes into Phosphatidylcholine or Phosphatidylethanolamine Liposomes

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Abstract

It is well known that flavonoids can chelate transition metals. Flavonoid-metal complexes exhibit a high antioxidative and therapeutic potential. However, the complexes are frequently hydrophobic ones and low soluble in water, which restricts their medical applications. Integration of these complexes into liposomes may increase their bioavailability and therapeutic effect. Here, we studied the interaction of quercetin-iron complexes with dimyristoylphosphatidylcholine (DMPC) or palmitoyl-oleoyl phosphatidylethanolamine (POPE) multilamellar liposomes. Differential scanning calorimetry (DSC) and freeze-fracture electron microscopy revealed that quercetin-iron complexes did not interact with liposomes. Quercetin however could penetrate lipid bilayers, when added to liposomes at a temperature above lipid melting. Iron cations added later penetrated into the lipid bilayers and produced complexes with quercetin in the liposomes. The quercetin-iron entry in POPE liposomes was improved when the suspension was heated above the temperature of the bilayer-hexagonal HII phase transition of the lipid. The approach proposed facilitates the integration of quercetin-iron complexes into liposomes and may promote their use in medicine.

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Abbreviations

DMPC:

1,2-Dimyristoyl-sn-glycero-3-phosphocholine

POPE:

1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine

DSC:

Differential scanning calorimetry

T p :

Temperature of pretransition

T m :

Temperature of the main phase transition

T h :

Temperature of transition from the bilayer to the hexagonal HII phase

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Acknowledgments

This study was supported by RFFI grants 13-04-97526 and 14-04-31308, Russian Federation.

Compliance with Ethical Standards

We believe that the presented research complies with the ethical rules applicable for the journal Applied Biochemistry and Biotechnology

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Authors and Affiliations

  1. Institute of Cell Biophysics, RAS, Pushchino, Moscow Region, 142290, Russia

    Yuri A. Kim

  2. Institute of Theoretical and Experimental Biophysics, RAS, Pushchino, Moscow Region, 142290, Russia

    Yury S. Tarahovsky & Svetlana M. Kuznetsova

  3. Tula State University, Tula, 300000, Russia

    Elena A. Yagolnik & Eugeny N. Muzafarov

Authors
  1. Yuri A. Kim
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  2. Yury S. Tarahovsky
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  3. Elena A. Yagolnik
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Correspondence to Yury S. Tarahovsky.

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Kim, Y.A., Tarahovsky, Y.S., Yagolnik, E.A. et al. Integration of Quercetin-Iron Complexes into Phosphatidylcholine or Phosphatidylethanolamine Liposomes. Appl Biochem Biotechnol 176, 1904–1913 (2015). https://doi.org/10.1007/s12010-015-1686-z

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