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Calcium Mobilization in Salicylic Acid-Induced Salvia miltiorrhiza Cell Cultures and Its Effect on the Accumulation of Rosmarinic Acid

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Abstract

Ca2+ serves as a second messenger in plant responses to different signals, and salicylic acid (SA) has been recognized as a signal mediating plant responses to many stresses. We recently found that SA treatment led to the cytoplasmic acidification of Salvia miltiorrhiza cells and alkalinization of extracellular medium. Here, we demonstrate that SA can rapidly induce Ca2+ mobilization in protoplasts, but the induction can be blocked with a channel blocker of either plasma or organellar membranes. Following SA, A 23187, or 10 mmol/L Ca2+ treatment, rosmarinic acid (RA) accumulation reached the highest level at 16 h, whereas the peak was found at 10 h if plasma membrane channel blockers were used. By contrast, the highest accumulation of RA occurred at 16 h when organellar channels were blocked, exhibiting the same tendency with SA-induced cells. In agreement with these observations, both phenylalanine ammonia-lyase (PAL) activity and its gene expression detected by real-time PCR also showed the same patterns. These results indicate that SA treatment firstly results in calcium release from internal stores, which in turn leads to PAL activity increase, RA accumulation, and a large amount of Ca2+ influx from apoplast after 10 h of SA induction.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31170274) and Academic Backbone Youth of Northwest A&F University (No. Z111020906).

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    Authors and Affiliations

    1. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, 712100, People’s Republic of China

      Hongbo Guo, Nan Zhu, Jun Liu, Xiaoru Zhang & Juane Dong

    2. Department of Biology, The University of British Columbia Okanagan, Kelowna, BC, Canada

      Hongbo Guo & Michael K. Deyholos

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    1. Hongbo Guo
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    Correspondence to Juane Dong.

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    Hongbo Guo and Nan Zhu contributed equally to this work.

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    Guo, H., Zhu, N., Deyholos, M.K. et al. Calcium Mobilization in Salicylic Acid-Induced Salvia miltiorrhiza Cell Cultures and Its Effect on the Accumulation of Rosmarinic Acid. Appl Biochem Biotechnol 175, 2689–2702 (2015). https://doi.org/10.1007/s12010-014-1459-0

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