Skip to main content
SpringerLink
Log in

Polygalacturonase Production by Calcium Alginate Immobilized Enterobacter aerogenes NBO2 Cells

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Bacterial cells of Enterobacter aerogenes NBO2 were entrapped in calcium alginate beads in order to enhance polygalacturonase production compared to free cells. The optimized condition of 5 % (w/v) sodium alginate concentration, agitation speed of 250 rpm, and 15 beads of calcium alginate with inoculum size of 4 % (v/v; 5.4 × 107 cells/ml) produced 23.48 U/mL of polygalacturonase compared to free cells of 18.54 U/ml. There was about 26.6 % increment in polygalaturonase production. However, in this study, there was 296.6 % of increment in polygalacturonase production after improvement parameters compared to before improvement parameters of calcium alginate bead immobilization cells (5.92 U/ml). This research has indicated that optimized physical parameters of calcium alginate bead immobilization cells have significantly enhanced the production of polygalacturonase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Ernesto, F. T., Tania, V. S., & Gustavo, V. G. (2006). Food Technology and Biotechnology, 44, 221–227.

    Google Scholar 

  2. Sarvamangala, R. P., & Agasar, D. (2006). Food Technology and Biotechnology, 44, 289–292.

    Google Scholar 

  3. Darah, I., Sumathi, G., Jain, K., & Lim, S. H. (2011). Journal of Biotechnology Research International. doi:10.4061/2011/897931.

    Google Scholar 

  4. Darah, I., Haritharan, W., & Lim, S. H. (2013). Journal of Pure Applied Microbiology, 7(4), 2541–2549.

    CAS  Google Scholar 

  5. Darah, I., Haritharan, W., & Lim, S. H. (2014). Process Biochemistry, 49, 660–667.

    Article  Google Scholar 

  6. Panesar, R., Panesar, P. S., Singh, R., & Bera, B. (2007). Journal of Food Process Engineering, 30, 472–484.

    Article  Google Scholar 

  7. Soares, M. M. C. N., Da Silva, R., & Gomes, E. (1999). Revista de Microbiology, 30, 299–303.

    CAS  Google Scholar 

  8. Adinarayan, K., Jyothi, B., & Ellaiah, P. (2005). AAPS Pharmacologica Science Technology, 6, 391–397.

    Article  Google Scholar 

  9. Sandai, D., Ibrahim, D., & Kassim, J. (2012). Indian Journal of Biotechnology, 6, 27–34.

    CAS  Google Scholar 

  10. Nelson, N. (1944). Journal of Biological Chemistry, 153, 375–380.

    CAS  Google Scholar 

  11. Somogyi, M. (1952). Journal of Biological Chemistry, 195, 19–21.

    CAS  Google Scholar 

  12. Darah, I., Sumathi, G., Jain, K., & Lim, S. H. (2011). Applied Biochemistry and Biotechnology, 165, 1682–1690.

    Article  CAS  Google Scholar 

  13. Mahajan, R., Gupta, V. K., & Sharma, J. (2010). Indian Journal Pharmacologica, 72, 223–228.

    CAS  Google Scholar 

  14. Konsoula, Z., & Liakopoulou-Kyriakides, L. M. (2004). Process Biochemistry, 39, 1745–1749.

    Article  Google Scholar 

  15. Zain, M. M., Kofli, N. T., & Yahya, S. R. S. (2011). IIUM Engineering Journal, 12, 11–19.

    Google Scholar 

  16. Talekar, S., & Chavare, S. (2012). Recent Research in Science and Technology, 4, 1–5.

    CAS  Google Scholar 

  17. Anwar, A., Ul-Qader, S. A., Raiz, A., Iqbal, S., & Azhar, A. (2009). World Applied Science Journal, 7, 1281–1286.

    CAS  Google Scholar 

Download references

Acknowledgement

The authors would like to thank the Universiti Sains Malaysia for awarding PRGS grant to Nisha, M. to complete her M. Sc. works.

Author information

Authors and Affiliations

  1. Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia

    I. Darah, M. Nisha & Sheh-Hong Lim

Authors
  1. I. Darah
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Nisha
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sheh-Hong Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sheh-Hong Lim.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Darah, I., Nisha, M. & Lim, SH. Polygalacturonase Production by Calcium Alginate Immobilized Enterobacter aerogenes NBO2 Cells. Appl Biochem Biotechnol 175, 2629–2636 (2015). https://doi.org/10.1007/s12010-014-1447-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-014-1447-4

Keywords

Search

Navigation