Abstract
Endophytic fungi of inner root bark of Taxus wallichiana var. mairei were investigated in order to find endophytes producing 10-DABIII (10-deacetyl baccatin III). Purified colonies were cultured in potato dextrose broth (PDB), and then the organic extracts from fungi were analyzed with HPLC, LC-MS, and 1H NMR. Of 102 fungal endophytes isolated from the inner root bark, only one strain named IRB54 can yield 10-DABIII but no taxol and baccatin III. In PDB culture medium, its productivity was 187.564 ug/l. Based on its morphological characteristics and molecular data, the IRB54 strain was identified as Trichoderma sp. The isolation of the fungus IRB54 yielding 10-DABIII will provide an alternative resource to manufacture taxol/taxotere via semi-synthesis and some useful clues for improving the understanding of taxane synthesis evolution.
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The research was supported by the Education Department of Henan Province and Science and Technology Department of Henan Province.
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Li, Y., Yang, J., Zhou, X. et al. Isolation and Identification of a 10-Deacetyl Baccatin-III-Producing Endophyte from Taxus wallichiana . Appl Biochem Biotechnol 175, 2224–2231 (2015). https://doi.org/10.1007/s12010-014-1422-0
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DOI: https://doi.org/10.1007/s12010-014-1422-0