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Enzymatic Activities in Different Strains Isolated from Healthy and Brittle Leaf Disease Affected Date Palm Leaves: Study of Amylase Production Conditions

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Abstract

The present study aimed to investigate and compare the enzymatic production of endophytic bacteria isolated from healthy and brittle leaf disease affected date palm leaves (pectinase, cellulase, lipase, and amylase). The findings revealed that the enzymatic products from the bacterial isolates of healthy date palm leaves were primarily 33% amylolytic enzyme, 33 % cellulase, 25 % pectinase, and 25 % lipase. The isolates from brittle leaf disease date palm leaves, on the other hand, were noted to produce 16 % amylolytic enzyme, 20 % cellulose, 50 % pectinase, and 50 % lipase. The effects of temperature and pH on amylase, pectinase, and cellulose activities were investigated. The Bacillus subtilis JN934392 strain isolated from healthy date palm leaves produced higher levels of amylase activity at pH 7. A Box Behnken Design (BBD) was employed to optimize amylase extraction. Maximal activity was observed at pH and temperature ranges of pH 6–6.5 and 37–39 °C, respectively. Under those conditions, amylase activity was noted to be attained 9.37 U/ml. The results showed that the enzyme was able to maintain more than 50 % of its activity over a temperature range of 50–80 °C, with an optimum at 70 °C. This bacterial amylase showed high activity compared to other bacteria, which provides support for its promising candidacy for future industrial application.

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Abbreviations

B.m:

Bacillus megaterium

B.sub:

Bacillus subtilis JN 934389

B.s:

Bacillus sonoensis

B.e:

Bacillus endophyticus

B.saf:

Bacillus safensis

A.a:

Arthrobacter agilis JN934384

M.b:

Microbacterium barkeri JN9343388

M.a:

Microbacterium aerolatum JN934392

P.s:

Pseudomonas sutzeri JN934394

S.n:

Staphylococcus napalensis

S.s:

Staphylococcus saccharolyticus

K.r :

Kocuria rosea

P.sp:

Pantoea sp.

E.h:

Enterobacter hormaechei

M.o:

Flavobacteriacea: Myroides odoratimimus

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Authors and Affiliations

  1. Laboratory of Plant Biotechnology Applied to Crop Improvement, Faculty of Science of Sfax, University of Sfax, 3038, Sfax, Tunisia

    Jrad Mouna, Ben Choba Ines, Drira Nourredine, Kadri Adel & Gharsallah Néji

  2. Faculty of Sciences of Sfax, Research Unit Environmental Toxicology and Microbiology - santé University ofSfax, Sfax, Tunisia

    Fendri Imen

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  1. Jrad Mouna
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  2. Fendri Imen
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  3. Ben Choba Ines
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  6. Gharsallah Néji
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Correspondence to Jrad Mouna.

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Mouna, J., Imen, F., Choba Ines, B. et al. Enzymatic Activities in Different Strains Isolated from Healthy and Brittle Leaf Disease Affected Date Palm Leaves: Study of Amylase Production Conditions. Appl Biochem Biotechnol 175, 2075–2086 (2015). https://doi.org/10.1007/s12010-014-1409-x

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  • DOI: https://doi.org/10.1007/s12010-014-1409-x

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