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Ultra-performance Liquid Chromatography Quadrupole Time-of-Flight MS for Identification of Electron Beam from Accelerator Degradation Products of Aflatoxin B1

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Abstract

Electron beam irradiation was proven to be a successful method in aflatoxin degradation in earlier researches. However, the exact nature of the result radiation products generated by the aflatoxins remains unknown. Based on ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF MS) analysis, the solution of aflatoxin B1 (AFB1) in acetonitrile irradiated by electron beam degraded to two kinds of major products. The doses employed were in the range of 0 (control) to 8.60 kGy. The absorbed doses were monitored with FWT-60-00 radio-chromic dosimeters. By using UPLC-Q-TOF MS, accurate masses and proposed molecular formula for the degradation products, 261.1233 m/z (C14H13O5) and 299.1104 m/z (C17H15O5), were obtained from low mass error and high matching properties. Structural formula for the radio-degradation products and the degradation pathways leading to the compounds were proposed, based on the molecular formula and MS-MS spectra. The results showed that electron beam (EB) irradiation is an effective method for degrading AFB1.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31401525) and the Natural Science Foundation of Jiangsu Province (BK20140156).

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu Province, People’s Republic of China

    Ruiqi Wang, Ruijie Liu, Ming Chang, Qingzhe Jin, Jianhua Huang, Yuanfa Liu & Xingguo Wang

  2. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, 163319, Heilongjiang Province, People’s Republic of China

    Ruiqi Wang

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  1. Ruiqi Wang
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  2. Ruijie Liu
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  3. Ming Chang
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  4. Qingzhe Jin
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  5. Jianhua Huang
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  6. Yuanfa Liu
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  7. Xingguo Wang
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Correspondence to Ruijie Liu.

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Ruiqi Wang and Ruijie Liu equally contributed to this work.

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Wang, R., Liu, R., Chang, M. et al. Ultra-performance Liquid Chromatography Quadrupole Time-of-Flight MS for Identification of Electron Beam from Accelerator Degradation Products of Aflatoxin B1 . Appl Biochem Biotechnol 175, 1548–1556 (2015). https://doi.org/10.1007/s12010-014-1377-1

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  • DOI: https://doi.org/10.1007/s12010-014-1377-1

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