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ROS and ABA Signaling Are Involved in the Growth Stimulation Induced by Low-Dose Gamma Irradiation in Arabidopsis Seedling

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Abstract

It has been well established that gamma rays at low doses have stimulatory effects on plant growth and development. However, our knowledge regarding the molecular mechanism underlying the growth stimulation remains limited. In this study, we report the role of reactive oxygen species (ROS) and abscisic acid (ABA) in the growth stimulation using irradiated Arabidopsis seeds. The results indicated that 50 Gy gamma irradiation presented maximal beneficial effects on germination index, root length, and fresh weight. The contents of hydrogen peroxide (H2O2) and activities of antioxidant enzymes under gamma irradiation were markedly higher than those of controls. ROS scavenging significantly suppressed the growth of the irradiated plants. Furthermore, endogenous ABA was induced under low-dose gamma irradiation. The growth stimulation and elevated H2O2 level were affected in the irradiated ABA-deficient mutant aba2-1 compared with the mutant control. Transcriptional expression analysis of selected genes revealed that several genes for ABA biosynthesis were upregulated, and the genes for ABA catabolic pathway and transport were differentially regulated in response to low-dose gamma irradiation. Our results suggest that ROS and ABA signaling play an essential role in the stimulatory effects of low-dose gamma irradiation and that ROS, as secondary molecules, mediate ABA signal transduction under irradiation in response to stress factors during plant growth.

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Acknowledgments

This work was funded by the National Natural Science Foundation of China (11405147, 11375154 and 31300163). The authors express great gratitude to Prof. Le Jie, Institute of Botany, Chinese Academy of Sciences, Beijing, China, for providing the ABA-deficient mutant (aba2-1) of A. thaliana.

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Authors and Affiliations

  1. Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou, 450052, China

    Wencai Qi, Hangbo Xu, Lin Wang & Zhen Jiao

  2. College of Life Science, Henan Normal University, Xinxiang, 453007, China

    Liang Zhang

  3. Luoyang Academy of Agriculture and Forestry Sciences, Luoyang, 471023, China

    Weisen Feng

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  1. Wencai Qi
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  2. Liang Zhang
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  3. Weisen Feng
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  4. Hangbo Xu
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  5. Lin Wang
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  6. Zhen Jiao
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Correspondence to Zhen Jiao.

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Wencai Qi and Liang Zhang contributed equally to this work.

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Suppl. Table 1

Accession numbers and primers of the genes used for real-time quantitative RT-PCR in this study. (DOCX 17 kb)

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Qi, W., Zhang, L., Feng, W. et al. ROS and ABA Signaling Are Involved in the Growth Stimulation Induced by Low-Dose Gamma Irradiation in Arabidopsis Seedling. Appl Biochem Biotechnol 175, 1490–1506 (2015). https://doi.org/10.1007/s12010-014-1372-6

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