Abstract
The callus and roots developed from the hypocotyl and cotyledon explants of the germinating seeds of Calotropis procera were grown in culture, and the proteins isolated from them (CP and RP) were evaluated for their efficacy in inhibiting edema formation induced by sub-plantar injection of carrageenan in the hind paw of rat. Intravenous administration of both CP and RP 30 min before inducing inflammation produced a dose-dependent inhibition of edema formation at 1 and 5 mg/kg doses. The extents of inhibition with these proteins ranged between 40 and 70 % at the doses included while the anti-inflammatory drug diclofenac produced 50 to 60 % inhibition at 5 mg/kg dose. The inhibitory effect with these proteins was accompanied by a dose-dependent reduction in the tissue levels of inflammatory mediators, tumor necrosis factor alpha (TNF-α) and prostaglandin E2 (PGE2), and oxidative stress markers namely glutathione and thiobarbituric acid-reactive substances and maintenance of tissue architecture. The present study shows that the proteins isolated from the differentiated and undifferentiated tissues derived from the germinating seeds have therapeutic application in the treatment of inflammatory conditions, and these tissues could be used as an alternative source to minimize variability of plant-derived formulations.
Similar content being viewed by others
References
Gogtay, N.J., Bhatt, H.A., Dalvi, S.S., & Kshirsagar, N.A. (2002). Drug Safety, 1005–1019.
de Ridder, S., van der Kooy, F., & Verpoorte, R. (2008). Journal of Ethnopharmacology, 120, 302–314.
Wealth of India. (1992). Raw materials (Vol 3, pp78–84). New Delhi: Information and Publication Directorate, CSIR.
Kumar, V. L., & Arya, S. (2006). Recent Progress in Medicinal Plants, 11, 373–388.
Kumar, V. L., & Roy, S. (2007). Mediators of Inflammation, 2007, 47523.
Choedon, T., Mathan, G., Arya, S., Kumar, V. L., & Kumar, V. (2006). World Journal of Gastroenterology, 12, 2517–2522.
Padhy, B. M., Srivastava, A., & Kumar, V. L. (2007). Journal of Ethnopharmacology, 113, 498–502.
Kumar, S., Dewan, S., Sangraula, H., & Kumar, V. L. (2001). Journal of Ethnopharmacology, 76, 115–118.
Dewan, S., Sangraula, H., & Kumar, V. L. (2000). Journal of Ethnopharmacology, 73, 307–311.
Dewan, S., Kumar, S., & Kumar, V. L. (2000). Indian Journal of Pharmacology, 32, 252.
Lima-Filho, J. V., Partriota, J. M., Silva, A. F., Filho, N. T., Pliveira, R. S., Alencar, N. M., et al. (2010). Journal of Ethnopharmacology, 129, 327–334.
Kumar, V. L., & Basu, N. (1994). Journal of Ethnopharmacology, 44, 123–125.
Alencar, N. M., Oliveira, J. S., Mesquita, R. O., Lima, M. W., Vale, M. R., Etchells, J. P., et al. (2006). Inflammation Research, 55, 559–564.
Soares, P. M., Lima, S. R., Matos, S. G., Andrade, M. M., Patrocinio, M. C., de Freitas, C.D., et al. (2005). Journal of Ethnopharmacology, 99, 125–129.
Kumar, V. L., Chaudhary, P., Ramos, M. V., Mohan, M., & Matos, M. P. V. (2011). Phytotherapy Research, 25, 1336–1341.
Freitas, A. P., Bitencourt, F. S., Brito, G. A., Alencar, N. M., Ribeiro, R. A., Lima-Junior, R. C., et al. (2012). Naunyn Schmeideberg’s Archives of Pharmacology, 385, 981–990.
Ramos, M. V., Viana, C. A., Silva, A. F., Freitas, C. D., Figueiredo, I. S., Oliveira, R. S., et al. (2012). Naunyn Schmeideberg’s Archives of Pharmacology, 385, 455–463.
Gad, H. A., El-Ahmady, S. H., Abou-Shoer, M. I., & Al-Azizi, M. M. (2013). Phytochemical Analysis, 24, 1–24.
Bourgaud, F., Gravot, A., Milesi, S., & Gontier, F. (2001). Plant Science, 161, 839–851.
Ikeuchi, M., Sugimoto, K., & Iwase, A. (2013). Plant Cell, 25, 3159–3173.
Sun, J., Xiao, J., Wang, X., Yuan, X., & Zhao, B. (2012). Biotechnology Letters, 34, 563–569.
Tripathi, P. K., Awasthi, S., Kanojiya, S., Tripathi, V., & Mishra, D. K. (2013). In Vitro Cellular & Developmental Biology - Plant, 49, 455–460.
Teixeira, F. M., Ramos, M. V., Soares, A. A., Oliveira, R. S., Almeida-Filho, L. C., Oliveira, J. S., et al. (2011). Process Biochemistry, 46, 1118–1124.
Winter, C. A., Risley, E. A., & Nuss, G. W. (1962). Proceedings of the Society for Experimental Biology and Medicine, 111, 544–547.
Ellman, G. L. (1959). Archives of Biochemistry and Biophysics, 82, 70–77.
Ohkawa, H., Ohishi, N., & Yagi, K. (1979). Analytical Biochemistry, 95, 351–358.
Chaturvedi, H. C., Jain, M., & Kidwai, N. R. (2007). Indian Journal of Experimental Biology, 45, 937–948.
Funk, C. D. (2001). Science, 294, 1871–1875.
Williams, T. J., & Morley, J. (1973). Nature, 246, 215–217.
Rekdal, O., Konopski, Z., Svendsen, J. S., Winberg, J. O., Espevik, T., & Osterud, B. (1994). Mediators of Inflammation, 3, 347–352.
Issekutz, A. C., & Movat, H. Z. (1982). American Journal of Pathology, 107, 300–309.
Beutler, B., & Cerami, A. (1986). Nature, 320, 584–588.
Pelkonen, O., Pasanen, M., Lindon, J. C., Chan, K., Zhao, L., & Deal, G. (2012). Journal of Ethnopharmacology, 140, 587–593.
Acknowledgements
MVR and CPSC acknowledge the financial support from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil, and Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP), Brazil.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kumar, V.L., Sharma, N., da Cósta Souza, I.C. et al. Proteins Derived from In Vitro Culture of the Callus and Roots of Calotropis procera Ameliorate Acute Inflammation in the Rat Paw. Appl Biochem Biotechnol 175, 1724–1731 (2015). https://doi.org/10.1007/s12010-014-1361-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-014-1361-9