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Production and Characterisation of Glycolipid Biosurfactant by Halomonas sp. MB-30 for Potential Application in Enhanced oil Recovery

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Abstract

Biosurfactant-producing Halomonas sp. MB-30 was isolated from a marine sponge Callyspongia diffusa, and its potency in crude oil recovery from sand pack column was investigated. The biosurfactant produced by the strain MB-30 reduced the surface tension to 30 mN m−1 in both glucose and hydrocarbon-supplemented minimal media. The critical micelle concentration of biosurfactant obtained from glucose-based medium was at 0.25 mg ml−1 at critical micelle dilution 1:10. The chemical structure of glycolipid biosurfactant was characterised by infrared spectroscopy and proton magnetic resonance spectroscopy. The emulsification activity of MB-30 biosurfactant was tested with different hydrocarbons, and 93.1 % emulsification activity was exhibited with crude oil followed by kerosene (86.6 %). The formed emulsion was stable for up to 1 month. To identify the effectiveness of biosurfactant for enhanced oil recovery in extreme environments, the interactive effect of pH, temperature and salinity on emulsion stability with crude oil and kerosene was evaluated. The stable emulsion was formed at and above pH 7, temperature >80 °C and NaCl concentration up to 10 % in response surface central composite orthogonal design model. The partially purified biosurfactant recovered 62 % of residual crude oil from sand pack column. Thus, the stable emulsifying biosurfactant produced by Halomonas sp. MB-30 could be used for in situ biosurfactant-mediated enhanced oil recovery process and hydrocarbon bioremediation in extreme environments.

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Acknowledgments

DA would like to acknowledge the Department of Science and Technology, New Delhi, Government of India for INSPIRE fellowship. JS is thankful to DBT for research grant.

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Authors and Affiliations

  1. Department of Microbiology, Bharathidasan University, Tiruchirappalli, 620 024, India

    Asha Dhasayan

  2. Department of Food Science and Technology, Pondicherry University, Puducherry, 605 014, India

    G. Seghal Kiran

  3. Department of Microbiology, Pondicherry University, R.V. Nagar, Kalapet, Puducherry, 605 014, India

    Joseph Selvin

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  1. Asha Dhasayan
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  2. G. Seghal Kiran
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Correspondence to Joseph Selvin.

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Dhasayan, A., Kiran, G.S. & Selvin, J. Production and Characterisation of Glycolipid Biosurfactant by Halomonas sp. MB-30 for Potential Application in Enhanced oil Recovery. Appl Biochem Biotechnol 174, 2571–2584 (2014). https://doi.org/10.1007/s12010-014-1209-3

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