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Anti-Bactericidal Properties of Stingray Dasyatis pastinaca Groups V, IIA, and IB Phospholipases A2: A Comparative Study

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Abstract

Group IIA secreted phospholipase A2 (group IIA sPLA2) is known to display potent Gram-positive bactericidal activity in vitro and in vivo. We have analyzed the bactericidal activity of the full set of native stingray and dromedary groups V, IIA, and IB sPLA2s on several Gram-positive and Gram-negative strains. The rank order potency among both marine and mammal sPLA2s against Gram-positive bacteria is group IIA > V > IB, whereas Gram-negative bacteria exhibited a much higher resistance. There is a synergic action of the sPLA2 with lysozyme when added to the bacteria culture prior to sPLA2.The bactericidal efficiency of groups V and IIA sPLA2s was shown to be dependent upon the presence of calcium ions and to a less extent Mg2+ ions and then a correlation could be made to its hydrolytic activity of membrane phospholipids. Importantly, we showed that stingray and dromedary groups V, IIA, and IB sPLA2s present no cytotoxicity after their incubation with MDA-MB-231cells. stingray groups V and IIA sPLA2s, like mammal ones, may be considered as future therapeutic agents against bacterial infections.

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Abbreviations

DrPLA2-IB:

Dromedary group IB phospholipase A2

DrPLA2-IIA:

Dromedary group IIA phospholipase A2

DrPLA2-V:

Dromedary group V phospholipase A2

sPLA2 :

Secreted phospholipase A2

SPLA2 :

Stingray phospholipase A2

SPLA2-IB:

Stingray group IB phospholipase A2

SPLA2-IIA:

Stingray group IIA phospholipase A2

SPLA2-V:

Stingray group V phospholipase A2

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Acknowledgments

This research project was supported by a grant from the “Research Center of the Female Scientific and Medical Colleges,” Deanship of Scientific Research, King Saud University.

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Authors and Affiliations

  1. Biochemistry Department, Science College, King Saud University, P.O Box 22452, Riyadh, 11495, Saudi Arabia

    Abir Ben Bacha

  2. Laboratory of Plant Biotechnology Applied to Crop Improvement, Faculty of Science of Sfax, University of Sfax, Sfax, 3038, Tunisia

    Abir Ben Bacha

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Correspondence to Abir Ben Bacha.

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Bacha, A.B. Anti-Bactericidal Properties of Stingray Dasyatis pastinaca Groups V, IIA, and IB Phospholipases A2: A Comparative Study. Appl Biochem Biotechnol 174, 1520–1534 (2014). https://doi.org/10.1007/s12010-014-1069-x

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