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Antibacterial Properties of Intestinal Phospholipase A2 from the Common Stingray Dasyatis pastinaca

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Abstract

Stingray phospholipase A2 group IIA (SPLA2-IIA) was recently isolated and purified to homogeneity from the intestine of the common stingray Dasyatis pastinaca, suggesting that this enzyme plays an important role in systemic bactericidal defense. The present study showed that SPLA2-IIA was highly bactericidal against Gram-positive bacteria with inhibition zones and minimal inhibitory concentration values in the range of 13–25 mm and 2–8 μg/ml, respectively, whereas Gram-negative bacteria exhibited a much higher resistance. The bactericidal efficiency of SPLA2-IIA was shown to be unaffected by high protein and salt concentrations, but dependent upon the presence of calcium ions, and then correlated to the hydrolytic activity of membrane phospholipids. Importantly, we showed that stingray phospholipase A2 group IIA presents no cytotoxicity after its incubation with MDA-MB-231 cells. SPLA2-IIA may be considered as a future therapeutic agent against bacterial infections.

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Abbreviations

SPLA2-IIA:

Stingray phospholipase A2 group IIA

sPLA2 :

Secreted phospholipase A2

SPLA2-IB:

Stingray phospholipase A2 group IB

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Acknowledgments

The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the research group project no: RGP-VPP-070.

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Authors and Affiliations

  1. Biochemistry Department, Science College, King Saud University, P.O. Box 22452, Riyadh, 11495, Saudi Arabia

    Abir Ben Bacha

  2. Laboratory of Plant Biotechnology Applied to Crop Improvement, Faculty of Science of Sfax, University of Sfax, Sfax, 3038, Tunisia

    Abir Ben Bacha

  3. Botany and Microbiology Department, Science College, King Saud University, P.O. Box 22452, Riyadh, 11495, Saudi Arabia

    Islem Abid

  4. LOEX/CUO-recherche, Hôpital du Saint-Sacrement, Centre Hospitalier Affilié de Québec, Université Laval, Québec, G1S 4L8, Canada

    Habib Horchani

  5. Département d’Ophtalmologie, Faculté de Médecine, Université Laval, Québec, G1S 4L8, Canada

    Habib Horchani

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  1. Abir Ben Bacha
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Correspondence to Abir Ben Bacha.

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Ben Bacha, A., Abid, I. & Horchani, H. Antibacterial Properties of Intestinal Phospholipase A2 from the Common Stingray Dasyatis pastinaca . Appl Biochem Biotechnol 168, 1277–1287 (2012). https://doi.org/10.1007/s12010-012-9856-8

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