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Biosynthesis of Silver Nanoparticles by Bacillus stratosphericus Spores and the Role of Dipicolinic Acid in This Process

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Abstract

Seeking for simple, rapid, and environmental-friendly routes to produce metal nanoparticles is quite attractive for various biotechnological applications. Biological synthesis method of silver nanoparticles has been found very promising due to their non-toxicity and simplicity. Here, the spores of Bacillus stratosphericus isolated from soil enriched with 30 % H2O2 were used for the production of silver nanoparticles. Furthermore, the possible mechanism of silver nanoparticle synthesis by the spores was elucidated for the first time. In this regard, dipicolinic acid (DPA) was shown to play a critical role as a nanoparticle-producing agent. UV–Vis absorption spectroscopy, X-ray diffraction technique, energy-dispersive spectroscopy, and transmission electron microscopy were used to characterize the nanoparticles. Unlike vegetative cells of B. stratosphericus, the spores and the purified DPA were capable of producing nanoparticles from silver nitrate (AgNO3). These biogenic nanoparticles, which were highly toxic against different pathogenic bacteria, showed mixed structures including spherical, triangular, cubic, and hexagonal with the approximate size between 2 and 20 nm in diameter. Our results illustrated the role of dipicolinic acid as a main factor for the synthesis of nanoparticles by the bacterial spores.

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Acknowledgments

The authors would like to thank the University of Isfahan for the financial support given to the PhD student for a training period in the Department of Biology and Microbiology.

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Authors and Affiliations

  1. Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, IR, 81746-73441, Iran

    Afrouzossadat Hosseini-Abari & Giti Emtiazi

  2. Interdisciplinary Program for Biochemical Engineering and Biotechnology, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-742, South Korea

    Sang-Hyuk Lee & Byung-Gee Kim

  3. Department of Chemical Engineering, College of Engineering, Dong-A University, Busan, Korea

    June-Hyung Kim

Authors
  1. Afrouzossadat Hosseini-Abari
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  2. Giti Emtiazi
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  3. Sang-Hyuk Lee
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  4. Byung-Gee Kim
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  5. June-Hyung Kim
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Correspondence to Giti Emtiazi or June-Hyung Kim.

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Hosseini-Abari, A., Emtiazi, G., Lee, SH. et al. Biosynthesis of Silver Nanoparticles by Bacillus stratosphericus Spores and the Role of Dipicolinic Acid in This Process. Appl Biochem Biotechnol 174, 270–282 (2014). https://doi.org/10.1007/s12010-014-1055-3

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  • DOI: https://doi.org/10.1007/s12010-014-1055-3

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