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Effect of Double-Step Steam Explosion Pretreatment in Bioethanol Production from Softwood

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Abstract

The study investigated the production of bioethanol from softwood, in particular pine wood chip. The steam explosion pretreatment was largely investigated, evaluating also the potential use of a double-step process to increase ethanol production through the use of both solid and liquid fraction after the pretreatment. The pretreatment tests were carried out at different conditions, determining the composition of solid and liquid fraction and steam explosion efficiency. The enzymatic hydrolysis was carried out with Ctec2 enzyme while the fermentation was carried out using Saccharomyces Cerevisiae yeast “red ethanol”. It was found that the best experimental result was obtained for a single-step pretreated sample (10.6 g of ethanol/100 g of initial biomass dry basis) for a 4.53 severity. The best double-step overall performance was equal to 8.89 g ethanol/100 g of initial biomass dry basis for a 4.27 severity. The enzymatic hydrolysis strongly depended on the severity of the pretreatment while the fermentation efficiency was mainly influenced by the concentration of the inhibitors. The ethanol enhancing potential of a double-step steam explosion could slightly increase the ethanol production compared to single-step potential.

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Abbreviations

SE:

Steam explosion

5-HMF:

5-hydroxymethil furfural

NREL:

National Renewable Energy Laboratory

DM:

Dry matter

HPLC:

High Performance Liquid Chromatography

AIR:

Acid-insoluble residue

AIL:

Acid-insoluble lignin

TGA:

Thermal-gravimetric analysis

WIS:

Water insoluble substrate

HY:

Hydrolysis yield

XMG:

Xylose mannose and galactose

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Acknowledgments

The authors would like to thank Novozymes and Fermentis for providing Cellic™Ctec2 and Red Ethanol®; furthermore, thanks to all the biofuel and biochemical laboratory team for the efforts and the helpful work done.

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Authors and Affiliations

  1. CIRIAF-Biomass Research Centre, University of Perugia, Via G. Duranti, 06125, Perugia, Italy

    Franco Cotana, Gianluca Cavalaglio, Mattia Gelosia, Valentina Coccia, Alessandro Petrozzi & Andrea Nicolini

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  1. Franco Cotana
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  2. Gianluca Cavalaglio
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  3. Mattia Gelosia
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  4. Valentina Coccia
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  5. Alessandro Petrozzi
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  6. Andrea Nicolini
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Correspondence to Gianluca Cavalaglio.

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Cotana, F., Cavalaglio, G., Gelosia, M. et al. Effect of Double-Step Steam Explosion Pretreatment in Bioethanol Production from Softwood. Appl Biochem Biotechnol 174, 156–167 (2014). https://doi.org/10.1007/s12010-014-1046-4

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  • DOI: https://doi.org/10.1007/s12010-014-1046-4

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