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Self-Cloning Significantly Enhances the Production of Catalase in Bacillus subtilis WSHDZ-01

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Abstract

The katA gene that encodes catalase (CAT) in Bacillus subtilis WSHDZ-01 was overexpressed in B. subtilis WB600 and B. subtilis WSHDZ-01. The CAT yield in both transformed strains was significantly improved compared to that in the wild-type WSHDZ-01 in shake flask culture. When cultured in a 3-L stirred tank reactor (STR), the recombinant CAT activity in B. subtilis WSHDZ-01 could be improved by 419 %, reaching up to 39,117 U/mL and was 8,149.4 U/mg dry cell weight, which is the highest activity reported in Bacillus sp. However, the recombinant CAT in B. subtilis WB600 cultured in a 3-L STR was not significantly improved by any of the common means for process optimization, and the highest CAT activity was 3,673.5 U/mg dry cell weight. The results suggest that self-cloning of the complete expression cassette in the original strain is a reasonable strategy to improve the yield of wild-type enzymes.

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Acknowledgments

We thank Dr. Dieter Jahn for the kind donation of plasmid pSTOP1622. This work was supported by grants from the National Natural Science Foundation of China (31370130), the Natural Science Foundation of Jiangsu Province (BK2011004), the Open Project Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China (KLIB-KF201006, KLIB-KF201106), the Author of National Excellent Doctoral Dissertation of PR China (FANEDD, 201256), the Program for New Century Excellent Talents in University (NCET-12-0876), the Fundamental Research Funds for the Central Universities (JUSRP51307A), the Self-determined Research Program of Jiangnan University (JUSRP211A25), and the 111 Project (111-2-06).

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  1. School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, China

    Sha Xu, Yaqiong Guo, Guocheng Du, Jingwen Zhou & Jian Chen

  2. Synergetic Innovation Center of Food Safety and Nutrition, 1800 Lihu Road, Wuxi, Jiangsu, 214122, China

    Jingwen Zhou & Jian Chen

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  1. Sha Xu
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Correspondence to Jingwen Zhou.

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Xu, S., Guo, Y., Du, G. et al. Self-Cloning Significantly Enhances the Production of Catalase in Bacillus subtilis WSHDZ-01. Appl Biochem Biotechnol 173, 2152–2162 (2014). https://doi.org/10.1007/s12010-014-1017-9

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