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Aptamer-based Biosensor for Detection of Phenylalanine at Physiological pH

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Abstract

A simple, sensitive aptamer-based biosensor for the detection of phenylalanine is developed using the electrochemical transduction method. For this proposed aptasensor, a 5-thiol-terminated aptamer is covalently attached onto a gold electrode. At the first time, the electrode was evaluated as an electrochemical aptasensor for determination of phenylalanine in aqueous solutions. This sensor was tested in a Tris–HCl buffer with physiological pH = 7.4 by cyclic voltammetry and differential pulse voltammetry. The detection limit and sensitivity of the modified electrode toward phenylalanine were estimated to be 1 nM (S/N = 3) and 0.367 μA nM−1, respectively. The linear range of the signal was observed between 1 and 10 nM of phenylalanine with 0.9914 correlation factor. The herein-described approach is expected to promote the exploitation of aptamer-based biosensors for protein assays in biochemical and biomedical studies.

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Acknowledgments

We gratefully acknowledge the support of this work by the Pasteur Institute of Iran and Drug Applied Research Center, Tabriz University of Medical Sciences. We especially thank Ali Khaneh-Zar and Hamid Shahbaz-Mohammadi.

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Authors and Affiliations

  1. Biochemistry Department, Genetic and Metabolism Group, Pasteur Institute of Iran, P.O. Box 13164, Tehran, Iran

    Eskandar Omidinia & Nasrin Shadjou

  2. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, 51664, Iran

    Mohammad Hasanzadeh

Authors
  1. Eskandar Omidinia
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  2. Nasrin Shadjou
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  3. Mohammad Hasanzadeh
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Correspondence to Eskandar Omidinia or Mohammad Hasanzadeh.

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Omidinia, E., Shadjou, N. & Hasanzadeh, M. Aptamer-based Biosensor for Detection of Phenylalanine at Physiological pH. Appl Biochem Biotechnol 172, 2070–2080 (2014). https://doi.org/10.1007/s12010-013-0656-6

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  • DOI: https://doi.org/10.1007/s12010-013-0656-6

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