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Antifungal Activity of Microgramma vacciniifolia Rhizome Lectin on Genetically Distinct Fusarium oxysporum f. sp. lycopersici Races

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Abstract

Fusarium oxysporum f. sp. lycopersici races 1, 2, and 3 deteriorate tomato crops since they cause a vascular wilt. Lectins are carbohydrate-binding proteins with hemagglutinating and antifungal activities. This work reports that Microgramma vacciniifolia rhizome lectin (MvRL) inhibits F. oxysporum f. sp. lycopersici race 3 growth (61 %) more intensely than of races 1 (55 %) and 2 (45 %). The hemagglutinating activity of MvRL was inhibited by glycoprotein preparations from mycelia of races 1, 2, and 3, and these data indicate that lectin carbohydrate-binding sites recognized glycosylated molecules from races. Inter-simple sequence repeat (ISSR) marker system showed that race 3 is genetically distinct from races 1 and 2, and thus the highest sensitiveness of F. oxysporum f. sp. lycopersici race 3 to MvRL may be due to molecular characteristics of this race.

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Acknowledgments

The authors express their gratitude to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for research grants and fellowships (LCBBC and PMGP), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE), and Ministério da Ciência, Tecnologia e Inovação (MCTI) for research grants. L.P. Albuquerque would like to thank CAPES and the Brazilian Ministry of Education (Programa de Reestruturação e Expansão das Universidades Federais - REUNI) for graduate scholarship. G.M.S. Santana would like to thank FACEPE for graduate scholarship. The authors are also deeply grateful to Maria Barbosa Reis da Silva for the technical assistance and Felix Nonnenmacher for English editing.

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Authors and Affiliations

  1. Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-420, Brazil

    Lidiane Pereira de Albuquerque, Giselly Maria de Sá Santana, Thiago Henrique Napoleão, Luana Cassandra Breitenbach Barroso Coelho, Márcia Vanusa da Silva & Patrícia Maria Guedes Paiva

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  1. Lidiane Pereira de Albuquerque
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  2. Giselly Maria de Sá Santana
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  3. Thiago Henrique Napoleão
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  4. Luana Cassandra Breitenbach Barroso Coelho
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  5. Márcia Vanusa da Silva
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  6. Patrícia Maria Guedes Paiva
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Correspondence to Patrícia Maria Guedes Paiva.

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de Albuquerque, L.P., de Sá Santana, G.M., Napoleão, T.H. et al. Antifungal Activity of Microgramma vacciniifolia Rhizome Lectin on Genetically Distinct Fusarium oxysporum f. sp. lycopersici Races. Appl Biochem Biotechnol 172, 1098–1105 (2014). https://doi.org/10.1007/s12010-013-0600-9

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