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Antibacterial and antifungal activities of Myracrodruon urundeuva heartwood

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Abstract

The aim of this work was to isolate a lectin from Myracrodruon urundeuva heartwood and to evaluate its antimicrobial activity against bacteria and fungi that attack plants, including woods. The lectin was isolated from heartwood through affinity chromatography on a chitin column monitored by hemagglutination assay. The lectin inhibited Gram-negative and Gram-positive bacteria and was more effective than antifungal Cercobin in growth inhibition of phytopathogenic fungi. The detected antimicrobial activity reveals the possible role of the lectin in the resistance of M. urundeuva heartwood against deteriorative biological agents. The M. urundeuva lectin is the first bioactive peptide found in heartwood, probably stored as a chemical protection against biodegradation.

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Abbreviations

CFU:

Colony forming units

F1 :

40–60% fraction

HA:

Hemagglutinating activity

MAC:

Minimal agglutinating concentration

MBC:

Minimal bactericide concentration

MIC:

Minimal inhibitory concentration

NA:

Nutrient Agar medium

NB:

Nutrient Broth medium

SHA:

Specific hemagglutinating activity

YNB:

Yeast nitrogen base medium

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Acknowledgments

The authors express their gratitude to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and to the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for research grants. Authors are deeply grateful to Maria Barbosa Reis da Silva (for the technical assistance) and to Msc. Gonçalo Mendes da Conceição (for the identification of the botanical material).

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Authors and Affiliations

  1. Departamento de Química Fundamental, CCEN, Universidade Federal de Pernambuco, Recife, PE, Brazil

    Roberto A. Sá & Lothar W. Bieber

  2. Departamento de Bioquímica, CCB, Universidade Federal de Pernambuco, Avenida Prof Moraes Rego s/n, 50670-420, Cidade Universitária, Recife, PE, Brazil

    Francis S. Gomes, Thiago H. Napoleão, Nataly D. L. Santos, Luana C. B. B. Coelho & Patrícia M. G. Paiva

  3. Departamento de Antibióticos, CCB, Universidade Federal de Pernambuco, Recife, PE, Brazil

    Carla M. L. Melo & Norma B. Gusmão

Authors
  1. Roberto A. Sá
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  2. Francis S. Gomes
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  3. Thiago H. Napoleão
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  4. Nataly D. L. Santos
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  5. Carla M. L. Melo
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  6. Norma B. Gusmão
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  7. Luana C. B. B. Coelho
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  8. Patrícia M. G. Paiva
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  9. Lothar W. Bieber
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Correspondence to Patrícia M. G. Paiva.

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Sá, R.A., Gomes, F.S., Napoleão, T.H. et al. Antibacterial and antifungal activities of Myracrodruon urundeuva heartwood. Wood Sci Technol 43, 85–95 (2009). https://doi.org/10.1007/s00226-008-0220-7

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  • DOI: https://doi.org/10.1007/s00226-008-0220-7

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