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Siderophore Production by Bacillus megaterium: Effect of Growth Phase and Cultural Conditions

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Abstract

Siderophore production by Bacillus megaterium was detected, in an iron-deficient culture medium, during the exponential growth phase, prior to the sporulation, in the presence of glucose; these results suggested that the onset of siderophore production did not require glucose depletion and was not related with the sporulation. The siderophore production by B. megaterium was affected by the carbon source used. The growth on glycerol promoted the very high siderophore production (1,182 μmol g−1 dry weight biomass); the opposite effect was observed in the presence of mannose (251 μmol g−1 dry weight biomass). The growth in the presence of fructose, galactose, glucose, lactose, maltose or sucrose, originated similar concentrations of siderophore (546–842 μmol g−1 dry weight biomass). Aeration had a positive effect on the production of siderophore. Incubation of B. megaterium under static conditions delayed and reduced the growth and the production of siderophore, compared with the incubation in stirred conditions.

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Acknowledgments

The authors thank Porto University/Totta Bank for their financial support through the project “Microbiological production of chelating agents” (Ref: 180). The authors also thank the Fundação para a Ciência e a Tecnologia (FCT) through the Portuguese Government for their financial support of this work through the grants Strategic project-LA23/2013-2014 (IBB) and PEST-C/EQB/LA0006/2011 (REQUIMTE). Manuela D. Machado gratefully acknowledges the postdoctoral (SFRH/BPD/72816/2010) grant from FCT.

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Authors and Affiliations

  1. Bioengineering Laboratory-CIETI, Chemical Engineering Department, ISEP-School of Engineering of Polytechnic Institute of Porto, Rua Dr António Bernardino de Almeida, 431, 4200-072, Porto, Portugal

    Sofia Santos, Manuela D. Machado & Eduardo V. Soares

  2. REQUIMTE-Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr Roberto Frias, s/n, 4200-465, Porto, Portugal

    Isabel F. F. Neto & Helena M. V. M. Soares

  3. IBB-Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal

    Manuela D. Machado & Eduardo V. Soares

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  1. Sofia Santos
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  2. Isabel F. F. Neto
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  3. Manuela D. Machado
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  4. Helena M. V. M. Soares
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Correspondence to Helena M. V. M. Soares or Eduardo V. Soares.

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Santos, S., Neto, I.F.F., Machado, M.D. et al. Siderophore Production by Bacillus megaterium: Effect of Growth Phase and Cultural Conditions. Appl Biochem Biotechnol 172, 549–560 (2014). https://doi.org/10.1007/s12010-013-0562-y

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  • DOI: https://doi.org/10.1007/s12010-013-0562-y

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