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Canine Parvovirus Type 2a (CPV-2a)-Induced Apoptosis in MDCK Involves Both Extrinsic and Intrinsic Pathways

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Abstract

The canine parvovirus type 2 (CPV-2) causes an acute disease in dogs. It has been found to induce cell cycle arrest and DNA damage leading to cellular lysis. In this paper, we evaluated the apoptotic potential of the “new CPV-2a” in MDCK cells and elucidated the mechanism of the induction of apoptosis. The exposure of MDCK cells to the virus was found to trigger apoptotic response. Apoptosis was confirmed by phosphatidylserine translocation, DNA fragmentation assays, and cell cycle analysis. Activation of caspases-3, -8, -9, and -12 and decrease in mitochondrial potential in CPV-2a-infected MDCK cells suggested that the CPV-2a-induced apoptosis is caspase dependent involving extrinsic, intrinsic, and endoplasmic reticulum pathways. Increase in p53 and Bax/Bcl2 ratio was also observed in CPV-2a-infected cells.

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Acknowledgments

The authors wish to thank the Director of Indian Veterinary Research Institute, Izatnagar for providing facilities. This work was carried out by a funding from NAIP under project code C4/C3001.

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Authors and Affiliations

  1. Molecular Biology Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India

    Juwar Doley, Lakshya Veer Singh, G. Ravi Kumar, Aditya Prasad Sahoo, Lovleen Saxena, Uttara Chaturvedi, Shikha Saxena, Rajiv Kumar, Prafull Kumar Singh, R. S. Rajmani, Lakshman Santra, S. K. Palia, S. Tiwari, D. R. Harish, Arvind Kumar, G. S. Desai, Smita Gupta, Shishir K. Gupta & A. K. Tiwari

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  1. Juwar Doley
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  2. Lakshya Veer Singh
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  4. Aditya Prasad Sahoo
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  9. Prafull Kumar Singh
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  12. S. K. Palia
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Correspondence to A. K. Tiwari.

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Doley, J., Singh, L.V., Kumar, G.R. et al. Canine Parvovirus Type 2a (CPV-2a)-Induced Apoptosis in MDCK Involves Both Extrinsic and Intrinsic Pathways. Appl Biochem Biotechnol 172, 497–508 (2014). https://doi.org/10.1007/s12010-013-0538-y

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  • DOI: https://doi.org/10.1007/s12010-013-0538-y

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