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Harnessing Indigenous Plant Seed Oil for the Production of Bio-fuel by an Oleaginous Fungus, Cunninghamella blakesleeana- JSK2, Isolated from Tropical Soil

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Abstract

Cunninghamella blakesleeana- JSK2, a gamma-linolenic acid (GLA) producing tropical fungal isolate, was utilized as a tool to evaluate the influence of various plant seed oils on biomass, oleagenicity and bio-fuel production. The fungus accumulated 26 % total lipid of their dry biomass (2 g/l) and 13 % of GLA in its total fatty acid. Among the various plant seed oils tested as carbon sources for biotransformation studies, watermelon oil had an effect on biomass and total lipid increasing up to 9.24 g/l and 34 % respectively. Sunflower, pumpkin, and onion oil increased GLA content between 15–18 %. Interestingly, an indigenous biodiesel commodity, Pongamia pinnata oil showed tremendous effect on fatty acid profile in C. blakesleeana- JSK2, when used as a sole source of carbon. There was complete inhibition of GLA from 13 to 0 % and increase in oleic acid content, one of the key components of biodiesel to 70 % (from 20 % in control). Our results suggest the potential application of indigenous plant seed oils, particularly P. pinnata oil, for the production of economically valuable bio-fuel in oleaginous fungi in general, and C. blakesleeana- JSK2, in particular.

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Acknowledgments

The authors acknowledge University Grant Council (UGC), India for funding research project entitled “Lipid profile of endophytic fungi: Identification of suitable strain for the production of commercially important omega fatty acids (EPA & DHA)”.

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Authors and Affiliations

  1. Department of Microbiology and Biotechnology, Jnana Bharathi Campus, Bangalore University, Bangalore, 560056,, Karnataka, India

    Sukrutha S. K & Savitha Janakiraman

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  1. Sukrutha S. K
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  2. Savitha Janakiraman
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Correspondence to Savitha Janakiraman.

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S. K, S., Janakiraman, S. Harnessing Indigenous Plant Seed Oil for the Production of Bio-fuel by an Oleaginous Fungus, Cunninghamella blakesleeana- JSK2, Isolated from Tropical Soil. Appl Biochem Biotechnol 172, 1027–1035 (2014). https://doi.org/10.1007/s12010-013-0531-5

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  • DOI: https://doi.org/10.1007/s12010-013-0531-5

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