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Effect of Physical and Chemical Properties of Oil Palm Empty Fruit Bunch, Decanter Cake and Sago Pith Residue on Cellulases Production by Trichoderma asperellum UPM1 and Aspergillus fumigatus UPM2

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Abstract

The effect of cultivation condition of two locally isolated ascomycetes strains namely Trichoderma asperellum UPM1 and Aspergillus fumigatus UPM2 were compared in submerged and solid state fermentation. Physical evaluation on water absorption index, solubility index and chemical properties of lignin, hemicellulose and cellulose content as well as the cellulose structure on crystallinity and amorphous region of treated oil palm empty fruit bunch (OPEFB) (resulted in partial removal of lignin), sago pith residues (SPR) and oil palm decanter cake towards cellulases production were determined. Submerged fermentation shows significant cellulases production for both strains in all types of substrates. Crystallinity of cellulose and its chemical composition mainly holocellulose components was found to significantly affect the total cellulase synthesis in submerged fermentation as the higher crystallinity index, and holocellulose composition will increase cellulase production. Treated OPEFB apparently induced the total cellulases from T. asperellum UPM1 and A. fumigatus UPM2 with 0.66 U/mg FPase, 53.79 U/mg CMCase, 0.92 U/mg β-glucosidase and 0.67 U/mg FPase, 47.56 U/mg and 0.14 U/mg β-glucosidase, respectively. Physical properties of water absorption and solubility for OPEFB and SPR also had shown significant correlation on the cellulases production.

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References

  1. Agensi Inovasi Malaysia (2011) National Biomass Strategy 2020: 21 November 2011.

  2. MPOB (Malaysian Palm Oil Board) (2009). Available from http://econ.mpob.gov.my/economy/Performance-130109.htm. Accessed 12 Jan 2009.

  3. Omar, R., Idris, A., Yunus, R., Khalid, K., & Aida Isma, M. I. (2011). Fuel, 90, 1536–1544.

    Article  CAS  Google Scholar 

  4. Yano, S., Murakami, K., Sawayama, S., Imou, K., & Yokoyama, S. (2009). Journal of The Japan Institute of Energy, 88, 377–391.

    Article  Google Scholar 

  5. Abdul Razak, M. N., Ibrahim, M. F., Yee, P. L., Hassan, M. A., & Abd-Aziz, S. (2012). Biotechnology and Bioprocess Engineering, 17, 547–555.

    Article  CAS  Google Scholar 

  6. Bujang, K. B. (1997). ICRO-UNESCO-MIRCEN Regional Training Course (pp. 4–16). Hat Yai: Prince of Songkhla University.

    Google Scholar 

  7. Bujang, K.B. and Ahmad. F.B. (2000). Country Report of Malaysia, International Sago Seminar. pp. 1–8.

  8. Prasad, S., Singh, A., & Joshi, H. C. (2007). Resources, Conservation and Recycling, 50, 1–39.

    Article  Google Scholar 

  9. Sánchez, Ó. J., & Cardona, C. A. (2008). Bioresource Technology, 99, 5270–5295.

    Article  Google Scholar 

  10. Singhania, R. R., Sukumaran, R. K., Patel, A. K., Larroche, C., & Pandey, A. (2010). Enzyme and Microbial Technology, 46, 541–549.

    Article  CAS  Google Scholar 

  11. Rabinovich, M. L., Melnick, M. S., & Bolobova, A. V. (2002). Biochemistry (Moscow), 67, 850–871.

    Article  CAS  Google Scholar 

  12. Umikalsom, M. S., Ariff, A. B., Zulkifli, H. S., Tong, C. C., Hassan, M. A., & Karim, M. I. A. (1997). Bioresource Technology, 62, 1–9.

    Article  CAS  Google Scholar 

  13. Abu Bakar, N. K., Abd-Aziz, S., Hassan, M. A., & Ghazali, F. M. (2010). Biotechnology, 9, 73–78.

    Article  CAS  Google Scholar 

  14. Mandel, M., & Weber, J. (1969). Advances in Chemistry, 95, 391–414.

    Article  Google Scholar 

  15. Wood, T. M., & Bhat, K. M. (1988). Methods for measuring cellulase activities. In W. A. Wood (Ed.), Methods in enzymology (pp. 87–112). New York: Academic.

    Google Scholar 

  16. Miller, G. L. (1959). Analytical Chemistry, 31, 426–428.

    Article  CAS  Google Scholar 

  17. Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Journal of Biological Chemistry, 193, 265–275.

    CAS  Google Scholar 

  18. AOAC:Official methods of analysis, Association of Official Analytical Chemists, Gaithersburg(16th ed.)(1997).

  19. Heinze, T., & Liebert, T. (2001). Progress in Polymer Science, 26, 1689–1762.

    Article  CAS  Google Scholar 

  20. Bledzki, A. K., Mamun, A. A., & Volk, J. (2010). Composites Part A: Applied Science and Manufacturing, 41, 480–488.

    Article  Google Scholar 

  21. Pandey, A. (1994). In A. Pandey (Ed.), Solid state fermentation (pp. 3–10). New Delhi: Wiley.

    Google Scholar 

  22. Zhang, Y. P., & Lynd, L. R. (2004). Biotechnology and Bioengineering, 88, 797–824.

    Article  CAS  Google Scholar 

  23. Tangerdy, R. P. (1996). Journal of Scientific and Industrial Research, 55, 313–316.

    Google Scholar 

  24. Dutta, T., Sahoo, R., Sengupta, R., Ray, S. S., Bhattacharjee, A., & Ghosh, S. (2008). Journal of Industrial Microbiology and Biotechnology, 35, 275–282.

    Article  CAS  Google Scholar 

  25. Elisashvili, V., Penninckx, M., Kachlishvili, E., Asatiani, M., & Kvesitadze, G. (2006). Enzyme and Microbial Technology, 38, 998–1004.

    Article  CAS  Google Scholar 

  26. Lopez, M. J., Vargas-García, M. C., Suárez-Estrella, F., Nichols, N. N., Dien, B. S., & Moreno, J. (2007). Enzyme and Microbial Technology, 40, 794–800.

    Article  CAS  Google Scholar 

  27. Mahadik, N. D., Puntambekar, U. S., Bastawde, K. B., Khire, J. M., & Gokhale, D. V. (2002). Process Biochemistry, 38, 715–721.

    Article  CAS  Google Scholar 

  28. Gusakov, A. V., Salanovich, T. N., Antonov, A. I., Ustinov, B. B., Okunev, O. N., Burlingame, R., et al. (2007). Biotechnology and Bioengineering, 97, 1028–1038.

    Article  CAS  Google Scholar 

  29. Wen, Z., Liao, W., & Chen, S. (2007). Process Biochemistry, 40, 3087–3094.

    Article  Google Scholar 

  30. Ahamed, A., & Vermette, P. (2008). Biochemical Engineering Journal, 40, 399–407.

    Article  CAS  Google Scholar 

  31. Brijwani, K. (2011). PhD, thesis Kansas State University.

  32. Aro, N., Pakula, T., & Penttilä, M. (2005). FEMS Microbiology Reviews, 29, 719–739.

    Article  CAS  Google Scholar 

  33. Ishizawa, C. I., Davis, M. F., Schell, D. F., & Johnson, D. K. (2007). Journal of Agricultural and Food Chemistry, 55, 2575–2581.

    Article  CAS  Google Scholar 

  34. Meshartree, M., Hogan, C. M., & Saddler, J. N. (1988). Biotechnology and Bioengineering, 31, 725–729.

    Article  CAS  Google Scholar 

  35. Cao, Y., & Tan, H. (2005). Enzyme and Microbial Technology, 36, 314–317.

    Article  CAS  Google Scholar 

  36. Evans, E. T., Wales, D. S., Bratt, R. P., & Sagar, B. F. (1992). Journal of General Microbiology, 138, 1639–1646.

    Article  CAS  Google Scholar 

  37. Pandey, A., Soccol, C. R., Rodriguez-Leon, J. A., & Poonan, N. (2001). In biotechnology, fundamental and applications (pg.220) 1sted. New Delhi: Asiatech.

    Google Scholar 

  38. Wolfgang, A. (2007). In: Enzyme and Industry; production and application. pp 35.Wiley-VCH.

  39. Rahardjo, Y. S. P., Jolink, F., Haemers, S., Tramper, J., & Rinzema, A. (2005). Biomolecular Engineering, 22, 133–139.

    Article  CAS  Google Scholar 

  40. Asdul, M. G., Ghule, J. E., Singh, R., Shaikh, H., Bastawdea, K. B., Gokhale, D. V., et al. (2004). Carbohydrate Polymers, 57, 67–72.

    Article  Google Scholar 

  41. Romero, M. D., Aguado, J., Gonzalez, L., & Ladero, M. (1999). Enzyme and Microbial Technology, 25, 244–250.

    Article  CAS  Google Scholar 

  42. Roslan, A. M., Hassan, M. A., Abd-Aziz, S., & Yee, P. L. (2009). International Journal of Agricultural Research, 4, 185–192.

    Article  CAS  Google Scholar 

  43. Kalogeris, E., Fountoukides, G., Kekos, D., & Macris, B. J. (1999). Bioresource Technology, 67, 313–315.

    Article  CAS  Google Scholar 

  44. Ibrahim, M. F., Razak, M. N. A., Phang, L. Y., Hassan, M. A., & Abd-Aziz, S. (2013). Applied Biochemistry and Biotechnology. doi:10.1007/s12010-013-0275-2.

    Google Scholar 

  45. Linggang, S., Phang, L. Y., Wasoh, S. B., & Abd-Aziz, S. (2012). Applied Biochemistry and Biotechnology, 167, 122–131.

    Article  CAS  Google Scholar 

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Acknowledgments

The authors would like to thank MyBrain 15 scholarship from the Ministry of Higher Education, Malaysia and the Research University Grant Scheme (RUGS) from Universiti Putra Malaysia for the financial support.

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Authors and Affiliations

  1. Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Zuraidah Zanirun, Ezyana Kamal Bahrin, Phang Lai-Yee, Mohd Ali Hassan & Suraini Abd-Aziz

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  1. Zuraidah Zanirun
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  2. Ezyana Kamal Bahrin
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  3. Phang Lai-Yee
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  5. Suraini Abd-Aziz
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Correspondence to Suraini Abd-Aziz.

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Zanirun, Z., Bahrin, E.K., Lai-Yee, P. et al. Effect of Physical and Chemical Properties of Oil Palm Empty Fruit Bunch, Decanter Cake and Sago Pith Residue on Cellulases Production by Trichoderma asperellum UPM1 and Aspergillus fumigatus UPM2. Appl Biochem Biotechnol 172, 423–435 (2014). https://doi.org/10.1007/s12010-013-0530-6

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