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Expression and Characterization of Recombinant GH11 Xylanase from Thermotolerant Streptomyces sp. SWU10

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Abstract

Xylans are major hemicellulose components of plant cell wall which can be hydrolyzed by xylanolytic enzymes. Three forms of endo-β-1,4-xylanases (XynSW1, XynSW2A, and XynSW2B) produced by thermotolerant Streptomyces sp. SWU10 have been reported. In the present study, we described the expression and characterization of the fourth xylanase enzyme from this bacteria, termed XynSW3. The gene containing 726 bp was cloned and expressed in Escherichia coli. The recombinant enzyme (rXynSW3) was purified from cell-free extract to homogeneity using Ni-affinity column chromatography. The apparent molecular mass of rXynSW3 was 48 kDa. Amino acid sequence analysis revealed that it belonged to a xylanase of glycoside hydrolase family 11. The optimum pH and temperature for enzyme activity were 5.5–6.5 and 50 °C, respectively. The enzyme was stable up to 40 °C and in wide pH ranges (pH 0.6–10.3). Xylan without arabinosyl side chain is the most preferable substrate for the enzyme. By using birch wood xylan as substrate, rXynSW3 produced several oligosaccharides in the initial stage of hydrolysis, and their levels increased with time, demonstrating that the enzyme is an endo-acting enzyme. The major products were xylobiose, triose, and tetraose. The rXynSW3 can be applied in several industries such as food, textile, and biofuel industries, and waste treatment.

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References

  1. Collins, T., Gerday, C., & Feller, G. (2005). FEMS Microbiology Review, 29, 3–23.

    Article  CAS  Google Scholar 

  2. Wilkie, K. C. B. (1979). Advances in Carbohydrate Chemistry and Biochemistry, 36, 215–264.

    Article  CAS  Google Scholar 

  3. Kormelink, F. J. M., & Voragen, A. G. J. (1993). Applied Microbiology and Biotechnology, 38, 688–695.

    Article  CAS  Google Scholar 

  4. Hector, R. E., Qureshi, N., Hughes, S. R., & Cotta, M. A. (2008). Applied Microbiology and Biotechnology, 80, 675–684.

    Article  CAS  Google Scholar 

  5. Beg, Q. A., Kapoor, M., Mahajan, G., & Hoondal, S. (2001). Applied Microbiology and Biotechnology, 56, 326–338.

    Article  CAS  Google Scholar 

  6. Kuhad, R. C., & Singh, A. (1993). Critical Reviews in Biotechnology, 13, 151–172.

    Article  CAS  Google Scholar 

  7. Collins, T., De Vos, D., Hoyoux, A., Savvides, S. N., Gerday, C., Van Beeumen, J., et al. (2005). Journal of Molecular Biology, 354, 425–435.

    Article  CAS  Google Scholar 

  8. Biely, P. (1985). Trends in Biotechnology, 3, 286–290.

    Article  CAS  Google Scholar 

  9. Biely, P., Vrsanská, M., & Claeyssens, M. (1991). European Journal of Biochemistry, 200, 157–163.

    Article  CAS  Google Scholar 

  10. Vrancken, K., Van Mellaert, L., & Anné, J. (2010). Methods in Molecular Biology, 668, 97–107.

    Article  CAS  Google Scholar 

  11. Deesukon, W., Nishimura, Y., Harada, N., Sakamoto, T., & Sukhumsirichart, W. (2011). Process Biochemistry, 46, 2255–2262.

    Article  CAS  Google Scholar 

  12. Deesukon, W., Nishimura, Y., Sakamoto, T., & Sukhumsirichart, W. (2013). Molecular Biotechnology, 54, 37–46.

    Article  CAS  Google Scholar 

  13. Somogyi, M. (1952). Journal of Biological Chemistry, 195, 19–23.

    CAS  Google Scholar 

  14. Li, N., Yang, P., Wang, Y., Luo, H., Meng, K., Wu, N., et al. (2008). Journal of Microbiology and Biotechnology, 18, 410–416.

    CAS  Google Scholar 

  15. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., et al. (1997). Nucleic Acids Research, 25, 3389–3402.

    Article  CAS  Google Scholar 

  16. Bendtsen, J. D., Nielsen, H., von Heijne, G., & Brunak, S. (2004). Journal of Molecular Biology, 340, 783–795.

    Article  Google Scholar 

  17. Laemmli, U. K. (1970). Nature, 227, 680–685.

    Article  CAS  Google Scholar 

  18. Blumenkrantz, N., & Asboe-Hansen, G. (1973). Analytical Biochemistry, 54, 484–489.

    Article  CAS  Google Scholar 

  19. Bentley, S. D., Chater, K. F., Cerdeno-Tarraga, A. M., Challis, G. L., Thomson, N. R., James, K. D., et al. (2002). Nature, 417, 141–147.

    Article  Google Scholar 

  20. Shareck, F., Roy, C., Yaguchi, M., Morosoli, R., & Kluepfel, D. (1991). Gene, 107, 75–82.

    Article  CAS  Google Scholar 

  21. Huang, J., Wang, G., & Xiao, L. (2006). Bioresource Technology, 97, 802–808.

    Article  CAS  Google Scholar 

  22. Mao, L., Meng, P., Zhou, C., Ma, L., Zhang, G., & Ma, Y. (2012). World Journal of Microbiology and Biotechnology, 28, 777–784.

    Article  CAS  Google Scholar 

  23. Qu, W., & Shao, W. (2011). Biotechnology Letters, 33, 1407–1416.

    Article  CAS  Google Scholar 

  24. Li, N., Shi, P., Yang, P., Wang, Y., Luo, H., Bai, Y., et al. (2009). Applied Biochemistry and Biotechnology, 159, 521–531.

    Article  CAS  Google Scholar 

  25. Kui, H., Luo, H., Shi, P., Bai, Y., Yuan, T., Wang, Y., et al. (2010). Applied Biochemistry and Biotechnology, 162, 953–965.

    Article  Google Scholar 

  26. Liu, W., Shi, P., Chen, Q., Yang, P., Wang, G., Wang, Y., et al. (2010). Applied Biochemistry and Biotechnology, 162, 1–12.

    Article  CAS  Google Scholar 

  27. Luo, H., Wang, Y., Li, J., Wang, H., Yang, J., Yang, Y., et al. (2009). Enzyme and Microbial Technology, 45, 126–133.

    Article  CAS  Google Scholar 

  28. Decelle, B., Tsang, A., & Storms, R. K. (2004). Current Genetics, 46, 166–175.

    Article  CAS  Google Scholar 

  29. Biely, P., Vrsanská, M., Tenkanen, M., & Kluepfel, D. (1997). Journal of Biotechnology, 57, 151–166.

    Article  CAS  Google Scholar 

  30. Vardakou, M., Katapodis, P., Samiotaki, M., Kekos, D., Panayotou, G., & Christakopoulos, P. (2003). International Journal of Biological Macromolecules, 33, 129–134.

    Article  CAS  Google Scholar 

  31. Pollet, A. (2010). Ph.D. Thesis, Katholieke Universiteit Leuven, Leuven.

  32. Prade, R. A. (1996). Biotechnology and Genetic Engineering Reviews, 13, 101–131.

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by grants from the Royal Golden Jubilee Ph.D. Program of Thailand Research Fund (PHD/0257/2549), Srinakharinwirot University (2557), and JSPS/NRCT (ACP program). The authors would like to thank Dr. Alfredo Villarroel for proofreading the article.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, 114 Sukhumvit 23, Bangkok, 10110, Thailand

    Wasana Sukhumsirichart, Warin Deesukon & Weeranuch Seesom

  2. Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, 599-8531, Japan

    Takuya Kawakami, Shotaro Matsumoto & Tatsuji Sakamoto

Authors
  1. Wasana Sukhumsirichart
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  2. Warin Deesukon
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  3. Takuya Kawakami
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  4. Shotaro Matsumoto
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  5. Weeranuch Seesom
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  6. Tatsuji Sakamoto
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Correspondence to Wasana Sukhumsirichart or Tatsuji Sakamoto.

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Sukhumsirichart, W., Deesukon, W., Kawakami, T. et al. Expression and Characterization of Recombinant GH11 Xylanase from Thermotolerant Streptomyces sp. SWU10. Appl Biochem Biotechnol 172, 436–446 (2014). https://doi.org/10.1007/s12010-013-0508-4

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