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The Effects of Selenium on Polyunsaturated Fatty Acids of Diasporangium jonesianum

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Abstract

Fungi had become the main resource of polyunsaturated fatty acids, especially linoleic acid. The research studied the effects and mechanism of selenium on polyunsaturated fatty acids of Diasporangium jonesianum. The results showed that selenium could significantly increase the yields of linoleic acid. In contrast, the growth and γ-linolenic acid yield of D. jonesianum was decreased under selenium treatments. Δ6-Fatty acid desaturase gene of D. jonesianum was investigated in this research. Sequence analysis indicated that this cDNA sequence encoded 235 amino acids. The conserved region of Δ6-fatty acid desaturase included three conserved histidine-rich domain, hydropathy profile, and was rich in disulfide bonds. This study showed that selenium may in discriminatively substitute S and incorporate selenium-amino acids into the desaturase that the conformation of enzyme active sites was impacted which leaded to the inhibition of the convert of linoleic acid to γ-linolenic acid and the over accumulation of linoleic acid. Selenium might enhance the fatty acid contents of fungi through influencing the desaturase structure.

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Acknowledgments

This research received financial support from a project funded by the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions, the National Key Technology Research and Development Program of China (2006BAD27B09), and the Program of Jiangsu Key Laboratory of Marine Biotechnology (2007HS012).

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Authors and Affiliations

  1. Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, People’s Republic of China

    Yong Jia, Xian-Yue Guan & Chuan-Chao Dai

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  1. Yong Jia
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  2. Xian-Yue Guan
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  3. Chuan-Chao Dai
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Correspondence to Chuan-Chao Dai.

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Jia, Y., Guan, XY. & Dai, CC. The Effects of Selenium on Polyunsaturated Fatty Acids of Diasporangium jonesianum . Appl Biochem Biotechnol 172, 561–569 (2014). https://doi.org/10.1007/s12010-013-0477-7

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  • DOI: https://doi.org/10.1007/s12010-013-0477-7

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